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Lee, Ja Yil
Biochemistry and Molecular Biophysics Lab.
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dc.citation.endPage 11135 -
dc.citation.number 26 -
dc.citation.startPage 11125 -
dc.citation.title JOURNAL OF BIOLOGICAL CHEMISTRY -
dc.citation.volume 292 -
dc.contributor.author Lee, Ja Yil -
dc.contributor.author Steinfeld, Justin B. -
dc.contributor.author Qi, Zhi -
dc.contributor.author Kwon, YoungHo -
dc.contributor.author Sung, Patrick -
dc.contributor.author Greene, Eric C. -
dc.date.accessioned 2023-12-21T22:10:25Z -
dc.date.available 2023-12-21T22:10:25Z -
dc.date.created 2017-07-31 -
dc.date.issued 2017-06 -
dc.description.abstract Homologous recombination plays key roles in double-strand break repair, rescue, and repair of stalled replication forks and meiosis. The broadly conserved Rad51/RecA family of recombinases catalyzes the DNA strand invasion reaction that takes place during homologous recombination. We have established single-stranded (ss) DNA curtain assays for measuring individual base triplet steps during the early stages of strand invasion. Here, we examined how base triplet stepping by RecA, Rad51, and Dmc1 is affected by DNA sequence imperfections, such as single and multiple mismatches, abasic sites, and single nucleotide insertions. Our work reveals features of base triplet stepping that are conserved among these three phylogenetic lineages of the Rad51/RecA family and also reveals lineage-specific behaviors reflecting properties that are unique to each recombinase. These findings suggest that Dmc1 is tolerant of single mismatches, multiple mismatches, and even abasic sites, whereas RecA and Rad51 are not. Interestingly, the presence of single nucleotide insertion abolishes recognition of an adjacent base triplet by all three recombinases. On the basis of these findings, we describe models for how sequence imperfections may affect base triplet recognition by Rad51/RecA family members, and we discuss how these models and our results may relate to the different biological roles of RecA, Rad51, and Dmc1. -
dc.identifier.bibliographicCitation JOURNAL OF BIOLOGICAL CHEMISTRY, v.292, no.26, pp.11125 - 11135 -
dc.identifier.doi 10.1074/jbc.M117.787614 -
dc.identifier.issn 0021-9258 -
dc.identifier.scopusid 2-s2.0-85021653273 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/22393 -
dc.identifier.url http://www.jbc.org/content/292/26/11125 -
dc.identifier.wosid 000404391000031 -
dc.language 영어 -
dc.publisher AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC -
dc.title Sequence imperfections and base triplet recognition by the Rad51/RecA family of recombinases -
dc.type Article -
dc.relation.journalWebOfScienceCategory Biochemistry & Molecular Biology -
dc.relation.journalResearchArea Biochemistry & Molecular Biology -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -

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