Full metadata record
DC Field | Value | Language |
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dc.citation.number | 5 | - |
dc.citation.startPage | 1088 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 18 | - |
dc.contributor.author | Kim, Kwang-Youn | - |
dc.contributor.author | Park, Kwang-Il | - |
dc.contributor.author | Kim, Sung-Hoon | - |
dc.contributor.author | Yu, Sun-Nyoung | - |
dc.contributor.author | Park, Sul-Gi | - |
dc.contributor.author | Kim, Young Woo | - |
dc.contributor.author | Seo, Young Kyo | - |
dc.contributor.author | Ma, Jin-Yeul | - |
dc.contributor.author | Ahn, Soon-Cheol | - |
dc.date.accessioned | 2023-12-21T22:13:59Z | - |
dc.date.available | 2023-12-21T22:13:59Z | - |
dc.date.created | 2017-07-05 | - |
dc.date.issued | 2017-05 | - |
dc.description.abstract | Recently, the interplay between autophagy and apoptosis has become an important factor in chemotherapy for cancer treatment. Inhibition of autophagy may be an effective strategy to improve the treatment of chemo-resistant cancer by consistent exposure to chemotherapeutic drugs. However, no reports have clearly elucidated the underlying mechanisms. Therefore, in this study, we assessed whether salinomycin, a promising anticancer drug, induces apoptosis and elucidated potential antitumor mechanisms in chemo-resistant prostate cancer cells. Cell viability assay, Western blot, annexin V/propidium iodide assay, acridine orange (AO) staining, caspase-3 activity assay, reactive oxygen species (ROS) production, and mitochondrial membrane potential were assayed. Our data showed that salinomycin alters the sensitivity of prostate cancer cells to autophagy. Pretreatment with 3-methyladenine (3-MA), an autophagy inhibitor, enhanced the salinomycin-induced apoptosis. Notably, salinomycin decreased phosphorylated of AKT and phosphorylated mammalian target of rapamycin (mTOR) in prostate cancer cells. Pretreatment with LY294002, an autophagy and PI3K inhibitor, enhanced the salinomycin-induced apoptosis by decreasing the AKT and mTOR activities and suppressing autophagy. However, pretreatment with PD98059 and SB203580, an extracellular signal-regulated kinases (ERK), and p38 inhibitors, suppressed the salinomycin-induced autophagy by reversing the upregulation of ERK and p38. In addition, pretreatment with N-acetyl-L-cysteine (NAC), an antioxidant, inhibited salinomycin-induced autophagy by suppressing ROS production. Our results suggested that salinomycin induces apoptosis, which was related to ROS-mediated autophagy through regulation of the PI3K/AKT/mTOR and ERK/p38 MAPK signaling pathways. | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.18, no.5, pp.1088 | - |
dc.identifier.doi | 10.3390/ijms18051088 | - |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.scopusid | 2-s2.0-85019959228 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/22317 | - |
dc.identifier.url | http://www.mdpi.com/1422-0067/18/5/1088 | - |
dc.identifier.wosid | 000404113900191 | - |
dc.language | 영어 | - |
dc.publisher | MDPI AG | - |
dc.title | Inhibition of autophagy promotes salinomycin-induced apoptosis via reactive oxygen species-mediated PI3K/AKT/mTOR and ERK/p38 MAPK-dependent signaling in human prostate cancer cells | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology; Chemistry, Multidisciplinary | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology; Chemistry | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | autophagy | - |
dc.subject.keywordAuthor | prostate cancer cells | - |
dc.subject.keywordAuthor | salinomycin | - |
dc.subject.keywordAuthor | reactive oxygen species | - |
dc.subject.keywordPlus | ACQUIRED-RESISTANCE | - |
dc.subject.keywordPlus | CARCINOMA-CELLS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | DOXORUBICIN | - |
dc.subject.keywordPlus | STATISTICS | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | DEATH | - |
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