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DC Field | Value | Language |
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dc.citation.startPage | 80 | - |
dc.citation.title | BIOTECHNOLOGY FOR BIOFUELS | - |
dc.citation.volume | 10 | - |
dc.contributor.author | Ainala, Satish Kumar | - |
dc.contributor.author | Seol, Eunhee | - |
dc.contributor.author | Kim, Jung Rae | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-21T22:37:24Z | - |
dc.date.available | 2023-12-21T22:37:24Z | - |
dc.date.created | 2017-06-14 | - |
dc.date.issued | 2017-03 | - |
dc.description.abstract | Background: Citrobacter amalonaticus Y19 is a good biocatalyst for production of hydrogen (H-2) from oxidation of carbon monoxide (CO) via the so-called water-gas-shift reaction (WGSR). It has a high H-2-production activity (23.83 mmol H-2 g(-1) cell h(-1)) from CO, and can grow well to a high density on various sugars. However, its H-2-production activity is expressed only when CO is present as an inducer and in the absence of glucose. Results: In order to avoid dependency on CO and glucose, in the present study, the native CO-inducible promoters of WGSR operons (CO dehydrogenase, CODH, and CODH-dependent hydrogenase, CO-hyd) in Y19 were carefully analyzed and replaced with strong and constitutive promoters screened from Y19. One engineered strain (Y19-PR1), selected from three positive ones after screening similar to 10,000 colonies, showed a similar CO-dependent H-2-production activity to that of wild-type Y19, without being affected by glucose and/or CO. Compared with wild-type Y19, transcription of the CODH operon in Y19-PR1 increased 1.5-fold, although that of the CO-hyd operon remained at a similar level. To enhance the activity of CO-Hyd in Y19-PR1, further modifications, including an increase in gene copy number and engineering of the 5' untranslated region, were attempted, but without success. Conclusions: Convenient recombinant Y19-PR1 that expresses CO-dependent H-2-production activity without being limited by CO and glucose was obtained. | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY FOR BIOFUELS, v.10, pp.80 | - |
dc.identifier.doi | 10.1186/s13068-017-0770-8 | - |
dc.identifier.issn | 1754-6834 | - |
dc.identifier.scopusid | 2-s2.0-85019017599 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/22218 | - |
dc.identifier.url | https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-017-0770-8 | - |
dc.identifier.wosid | 000397486900002 | - |
dc.language | 영어 | - |
dc.publisher | BIOMED CENTRAL LTD | - |
dc.title | Citrobacter amalonaticus Y19 for constitutive expression of carbon monoxide-dependent hydrogen-production machinery | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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