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DC Field | Value | Language |
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dc.citation.endPage | 297 | - |
dc.citation.startPage | 293 | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 93 | - |
dc.contributor.author | Lee, Chang Yeol | - |
dc.contributor.author | Park, Ki Soo | - |
dc.contributor.author | Jung, Yun Kyung | - |
dc.contributor.author | Park, Hyun Gyu | - |
dc.date.accessioned | 2023-12-21T22:09:11Z | - |
dc.date.available | 2023-12-21T22:09:11Z | - |
dc.date.created | 2016-11-18 | - |
dc.date.issued | 2017-07 | - |
dc.description.abstract | A novel label-free system for the sensitive fluorescent detection of deoxyribonuclease I (DNase I) activity has been developed by utilizing DNA-templated silver nanocluster/graphene oxide (DNA-AgNC/GO) nanocomposite. AgNC is first synthesized around C-rich template DNA and the resulting DNA-AgNC binds to GO through the interaction between the extension DNA and GO. The resulting DNA-AgNC/GO would show quite reduced fluorescence signal because the fluorescence from DNA-AgNCs is quenched by GO. In the presence of DNase I, however, it degrades the DNA strand within DNA/RNA hybrid duplex probe employed in this study, consequently releasing RNA which is complementary to the extension DNA. The released free RNA then extracts DNA-AgNC from GO by hybridizing with the extension DNA bound to GO. This process would restore the quenched fluorescence, emitting highly enhanced fluorescence signal. By employing this assay principle, DNase I activity was reliably identified with a detection limit of 0.10. U/ml which is lower than those from previous fluorescence-based methods. Finally, the practical capability of this assay system was successfully demonstrated by its use to determine DNase I activity in bovine urine. | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.93, pp.293 - 297 | - |
dc.identifier.doi | 10.1016/j.bios.2016.08.073 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.scopusid | 2-s2.0-84994103438 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/21957 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0956566316308296 | - |
dc.identifier.wosid | 000399259000041 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | A label-free fluorescent assay for deoxyribonuclease I activity based on DNA-templated silver nanocluster/graphene oxide nanocomposite | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology | - |
dc.relation.journalResearchArea | Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Deoxyribonuclease I | - |
dc.subject.keywordAuthor | RNA/DNA hybrid | - |
dc.subject.keywordAuthor | DNA-templated silver nanocluster | - |
dc.subject.keywordAuthor | Graphene oxide | - |
dc.subject.keywordAuthor | Nanocomposite | - |
dc.subject.keywordAuthor | Fluorescent enzyme assay | - |
dc.subject.keywordPlus | TRANSIENT MYOCARDIAL-ISCHEMIA | - |
dc.subject.keywordPlus | GRAPHENE OXIDE | - |
dc.subject.keywordPlus | AG NANOCLUSTERS | - |
dc.subject.keywordPlus | SERUM | - |
dc.subject.keywordPlus | BIOSENSOR | - |
dc.subject.keywordPlus | MARKER | - |
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