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DC Field | Value | Language |
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dc.citation.startPage | 95 | - |
dc.citation.title | BIOTECHNOLOGY FOR BIOFUELS | - |
dc.citation.volume | 9 | - |
dc.contributor.author | Sekar, Balaji Sundara | - |
dc.contributor.author | Seol, Eunhee | - |
dc.contributor.author | Raj, Subramanian Mohan | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-21T23:48:23Z | - |
dc.date.available | 2023-12-21T23:48:23Z | - |
dc.date.created | 2017-02-19 | - |
dc.date.issued | 2016-04 | - |
dc.description.abstract | Background: Fermentative hydrogen (H-2) production suffers from low carbon-to-H-2 yield, to which problem, co-production of ethanol and H-2 has been proposed as a solution. For improved co-production of H-2 and ethanol, we developed Escherichia coli BW25113 Delta hycA Delta hyaAB Delta hybBC Delta ldhA Delta frdAB Delta pta-ackA Delta pfkA (SH8*) and overexpressed Zwf and Gnd, the key enzymes in the pentose-phosphate (PP) pathway (SH8*_ZG). However, the amount of accumulated pyruvate, which was significant (typically 0.20 mol mol(-1) glucose), reduced the co-production yield. Results: In this study, as a means of reducing pyruvate accumulation and improving co-production of H-2 and ethanol, we developed and studied E. coli SH9*_ZG with functional acetate production pathway for conversion of acetyl-CoA to acetate (pta-ackA(+)). Our results indicated that the presence of the acetate pathway completely eliminated pyruvate accumulation and substantially improved the co-production of H-2 and ethanol, enabling yields of 1.88 and 1.40 mol, respectively, from 1 mol glucose. These yields, significantly, are close to the theoretical maximums of 1.67 mol H-2 and 1.67 mol ethanol. To better understand the glycolytic flux distribution, glycolytic flux prediction and RT-PCR analyses were performed. Conclusion: The presence of the acetate pathway along with activation of the PP pathway eliminated pyruvate accumulation, thereby significantly improving co-production of H-2 and ethanol. Our strategy is applicable to anaerobic production of biofuels and biochemicals, both of which processes demand high NAD(P)H. | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY FOR BIOFUELS, v.9, pp.95 | - |
dc.identifier.doi | 10.1186/s13068-016-0510-5 | - |
dc.identifier.issn | 1754-6834 | - |
dc.identifier.scopusid | 2-s2.0-84964658685 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/21657 | - |
dc.identifier.url | http://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-016-0510-5 | - |
dc.identifier.wosid | 000375095800001 | - |
dc.language | 영어 | - |
dc.publisher | BIOMED CENTRAL LTD | - |
dc.title | Co-production of hydrogen and ethanol by pfkA-deficient Escherichia coli with activated pentose-phosphate pathway: reduction of pyruvate accumulation | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Biohydrogen | - |
dc.subject.keywordAuthor | Co-production of hydrogen and ethanol | - |
dc.subject.keywordAuthor | Glycolysis | - |
dc.subject.keywordAuthor | Pentose-phosphate pathway | - |
dc.subject.keywordAuthor | NADPH production | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.subject.keywordPlus | 3-HYDROXYPROPIONIC ACID | - |
dc.subject.keywordPlus | KLEBSIELLA-PNEUMONIAE | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | GLYCEROL | - |
dc.subject.keywordPlus | GLUCOSE | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | FERMENTATION | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | STRAINS | - |
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