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DC Field | Value | Language |
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dc.citation.endPage | 1255 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1247 | - |
dc.citation.title | ACS SYNTHETIC BIOLOGY | - |
dc.citation.volume | 5 | - |
dc.contributor.author | Lim, Hyun Gyu | - |
dc.contributor.author | Noh, Myung Hyun | - |
dc.contributor.author | Jeong, Jun Hong | - |
dc.contributor.author | Park, Sunghoon | - |
dc.contributor.author | Jung, Gyoo Yeol | - |
dc.date.accessioned | 2023-12-21T23:07:17Z | - |
dc.date.available | 2023-12-21T23:07:17Z | - |
dc.date.created | 2017-02-19 | - |
dc.date.issued | 2016-11 | - |
dc.description.abstract | 3-Hydroxypropionic acid (3-HP) can be biologically produced from glycerol by two consecutive enzymatic reactions, dehydration of glycerol to 3-hydroxypropionaldehyde (3-HPA) and oxidation of 3-HPA. The pathway has been proved efficient, but imbalance between the rates of the two enzymatic reactions often results in the accumulation of the toxic 3-HPA, which severely reduces cell viability and 3-HP production. In this study, we used UTR engineering to maximally increase the activities of glycerol dehydratase (GDHt) and aldehyde dehydrogenase (ALDH) for the high conversion of glycerol to 3-HP. Thereafter, the activity of GDHt was precisely controlled to avoid the accumulation of 3-HPA by varying expression of dhaB1, a gene encoding a main subunit of GDHt. The optimally balanced E. coil HGL_DBK4 showed a substantially enhanced 3-HP titer and productivity compared with the parental strain. The yield on glycerol, 0.97 g 3HP/g glycerol, in a fed-batch experiment, was the highest ever reported. | - |
dc.identifier.bibliographicCitation | ACS SYNTHETIC BIOLOGY, v.5, no.11, pp.1247 - 1255 | - |
dc.identifier.doi | 10.1021/acssynbio.5b00303 | - |
dc.identifier.issn | 2161-5063 | - |
dc.identifier.scopusid | 2-s2.0-85017356007 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/21653 | - |
dc.identifier.url | http://pubs.acs.org/doi/abs/10.1021/acssynbio.5b00303 | - |
dc.identifier.wosid | 000388428100008 | - |
dc.language | 영어 | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Optimum Rebalancing of the 3-Hydroxypropionic Acid Production Pathway from Glycerol in Escherichia coli | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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