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DC Field | Value | Language |
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dc.citation.number | 6 | - |
dc.citation.startPage | 064108 | - |
dc.citation.title | BIOMICROFLUIDICS | - |
dc.citation.volume | 10 | - |
dc.contributor.author | Han, Minsub | - |
dc.contributor.author | Kim, Byoung Choul | - |
dc.contributor.author | Matsuoka, Toshiki | - |
dc.contributor.author | Thouless, MD | - |
dc.contributor.author | Takayama, Shuichi | - |
dc.date.accessioned | 2023-12-21T23:07:24Z | - |
dc.date.available | 2023-12-21T23:07:24Z | - |
dc.date.created | 2016-12-20 | - |
dc.date.issued | 2016-11 | - |
dc.description.abstract | This paper uses computer simulations to reveal unprecedented details about linearization of deoxyribonucleic acid (DNA) inside dynamic nanochannels that can be repeatedly widened and narrowed. We first analyze the effect of rate of channel narrowing on DNA linearization dynamics. Quick (similar to 0.1 s) narrowing of nanoscale channels results in rapid overstretching of the semi-flexible chain followed by a slower (similar to 0.1-10 s) relaxation to an equilibrium extension. Two phenomena that induce linearization during channel narrowing, namely, elongational-flow and confinement, occur simultaneously, regardless of narrowing speed. Interestingly, although elongational flow is a minimum at the mid-point of the channel and increases towards the two ends, neither the linearization dynamics nor the degree of DNA extension varies significantly with the center-of-mass of the polymer projected on the channel axis. We also noticed that there was a significant difference in time to reach the equilibrium length, as well as the degree of DNA linearization at short times, depending on the initial conformation of the biopolymer. Based on these observations, we tested a novel linearization protocol where the channels are narrowed and widened repeatedly, allowing DNA to explore multiple conformations. Repeated narrowing and widening, something uniquely enabled by the elastomeric nanochannels, significantly decrease the time to reach the equilibrium-level of stretch when performed within periods comparable to the chain relaxation time and more effectively untangle chains into more linearized biopolymers. Published by AIP Publishing. | - |
dc.identifier.bibliographicCitation | BIOMICROFLUIDICS, v.10, no.6, pp.064108 | - |
dc.identifier.doi | 10.1063/1.4967963 | - |
dc.identifier.issn | 1932-1058 | - |
dc.identifier.scopusid | 2-s2.0-84999636160 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/21029 | - |
dc.identifier.url | http://aip.scitation.org/doi/abs/10.1063/1.4967963?journalCode=bmf | - |
dc.identifier.wosid | 000390113600009 | - |
dc.language | 영어 | - |
dc.publisher | AMER INST PHYSICS | - |
dc.title | Dynamic simulations show repeated narrowing maximizes DNA linearization in elastomeric nanochannels | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods; Biophysics; Nanoscience & Nanotechnology; Physics, Fluids & Plasmas | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology; Biophysics; Science & Technology - Other Topics; Physics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | SINGLE | - |
dc.subject.keywordPlus | LENGTH | - |
dc.subject.keywordPlus | CONFORMATION | - |
dc.subject.keywordPlus | EXTENSION | - |
dc.subject.keywordPlus | MOLECULES | - |
dc.subject.keywordPlus | MODELS | - |
dc.subject.keywordPlus | TIME | - |
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