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김용환

Kim, Yong Hwan
Enzyme and Protein Engineering Lab.
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dc.citation.endPage 400 -
dc.citation.number 4 -
dc.citation.startPage 397 -
dc.citation.title JOURNAL OF FERMENTATION AND BIOENGINEERING -
dc.citation.volume 83 -
dc.contributor.author Kim, Yong Hwan -
dc.contributor.author Kim, Ji Hyeon -
dc.contributor.author Yoo, Young Je -
dc.date.accessioned 2023-12-22T12:38:10Z -
dc.date.available 2023-12-22T12:38:10Z -
dc.date.created 2016-12-06 -
dc.date.issued 1997 -
dc.description.abstract Effects of various organic solvents and polyethylene glycol (PEG) on the secretion of peroxidase from carrot hairy roots were investigated. The peroxidase activity in culture broth was significantly enhanced without adverse effects on root growth when PEG was employed while other organic permeabilizing agents exerted harmful effects on root growth. PEG with a molecular weight of over 6,000 g/mol enhanced the secretion of peroxidase dramatically. Since PEG did not induce the formation of pores in the root cell membrane as confirmed by staining using Neutral Red, it was thought that the mechanism of secretion enhancement of peroxidase by PEG is different from that of other organic solvents which make the cell membrane permeable. Since PEG does not adversely affect the root growth, PEG can be applied to the repeated use of root cells for production of peroxidase -
dc.identifier.bibliographicCitation JOURNAL OF FERMENTATION AND BIOENGINEERING, v.83, no.4, pp.397 - 400 -
dc.identifier.doi 10.1016/S0922-338X(97)80151-1 -
dc.identifier.issn 0922-338X -
dc.identifier.scopusid 2-s2.0-0343852899 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/20913 -
dc.identifier.url http://www.sciencedirect.com/science/article/pii/S0922338X97801511 -
dc.identifier.wosid A1997XD61900018 -
dc.language 영어 -
dc.publisher The Society of Bioscience and Bioengineering Japan/Seibutsu Kogakkai -
dc.title Enhanced secretion of peroxidase from carrot hairy roots using polyethylene glycol -
dc.type Article -
dc.description.journalRegisteredClass scopus -

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