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DC Field | Value | Language |
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dc.citation.endPage | 570 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 565 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 426 | - |
dc.contributor.author | Lee, Ja Yil | - |
dc.contributor.author | Wang, Feng | - |
dc.contributor.author | Fazio, Teresa | - |
dc.contributor.author | Wind, Shalom | - |
dc.contributor.author | Greene, Eric C. | - |
dc.date.accessioned | 2023-12-22T04:39:22Z | - |
dc.date.available | 2023-12-22T04:39:22Z | - |
dc.date.created | 2016-09-30 | - |
dc.date.issued | 2012-10 | - |
dc.description.abstract | We report a new approach to probing DNA-protein interactions by combining optical tweezers with a high-throughput DNA curtains technique. Here we determine the forces required to remove the individual lipid-anchored DNA molecules from the bilayer. We demonstrate that DNA anchored to the bilayer through a single biotin-streptavidin linkage withstands similar to 20 pN before being pulled free from the bilayer, whereas molecules anchored to the bilayer through multiple attachment points can withstand >= 65 pN; access to this higher force regime is sufficient to probe the responses of protein-DNA interactions to force changes. As a proof-of-principle, we concurrently visualized DNA-bound fluorescently-tagged RNA polymerase while simultaneously stretching the DNA molecules. This work presents a step towards a powerful experimental platform that will enable concurrent visualization of DNA curtains while applying defined forces through optical tweezers. (C) 2012 Elsevier Inc. All rights reserved | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.426, no.4, pp.565 - 570 | - |
dc.identifier.doi | 10.1016/j.bbrc.2012.08.127 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.scopusid | 2-s2.0-84867227467 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/20498 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0006291X12016956 | - |
dc.identifier.wosid | 000310101000021 | - |
dc.language | 영어 | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Measuring intermolecular rupture forces with a combined TIRF-optical trap microscope and DNA curtains | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | DNA curtains | - |
dc.subject.keywordAuthor | Optical trap | - |
dc.subject.keywordAuthor | Single-molecule | - |
dc.subject.keywordAuthor | Combination | - |
dc.subject.keywordAuthor | DNA pulling | - |
dc.subject.keywordAuthor | Rupture force | - |
dc.subject.keywordPlus | SINGLE-MOLECULE FLUORESCENCE | - |
dc.subject.keywordPlus | RNA-POLYMERASE | - |
dc.subject.keywordPlus | TWEEZERS | - |
dc.subject.keywordPlus | SPECTROSCOPY | - |
dc.subject.keywordPlus | ELASTICITY | - |
dc.subject.keywordPlus | NUCLEOSOMES | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | CHROMATIN | - |
dc.subject.keywordPlus | BIOLOGY | - |
dc.subject.keywordPlus | PROTEIN | - |
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