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김용환

Kim, Yong Hwan
Enzyme and Protein Engineering Lab.
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Optimized refolding and characterization of S-peroxidase (CWPO_C of Populus alba) expressed in E.coli

Author(s)
Pham, Le Thanh MaiKim, Su JinSong, Bong KeunKim, Yong Hwan
Issued Date
2011-12
DOI
10.1016/j.pep.2011.08.003
URI
https://scholarworks.unist.ac.kr/handle/201301/20367
Fulltext
http://www.sciencedirect.com/science/article/pii/S1046592811002014
Citation
PROTEIN EXPRESSION AND PURIFICATION, v.80, no.2, pp.268 - 273
Abstract
Cationic cell wall peroxidase (CWPO_C) from poplar tree (Populus alba L) was heterologously expressed in Escherichia coli as an inclusion body. The insoluble inclusion body was solubilized and reactivated via a refolding procedure. The condition for this procedure was optimized by varying the refolding pH, and the concentrations of the oxidizing agent (GSSG), denaturing agent (GndCl), and hemin, respectively. The optimal conditions for refolding CWPO_C were 100 mM Tris-HCl at pH 8.5, 0.6 mM GSSG, 5 mu M hemin, 0.6 M GndCl and 5 mM CaCl2. The fact that the absorbance spectrum was identical to that of wild CWPO_C from poplar tree suggests that the protein folding, heme insertion and iron coordination were correctly archived. The binding affinity and turnover rate values of refolded CWPO_C were compared with those of HRP_C. k(cat)/K-m for sinapyl alcohol of CWPO_C was over 170 times higher than that of HRP_C, on the while k(cat)/K-m for coniferyl alcohol showed similar values for both peroxidase. The kinetic parameters showed that refolded CWPO_C possesses a very unique property of S-peroxidase, preferentially oxidizes sinapyl alcohol rather than coniferyl alcohol. The successful expression of CWPO_C in E. coli provides a valuable tool to elucidate the structure and functional relationship of S-peroxidase, which plays an important role in the lignification of angiosperm woody plant cell walls.
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
ISSN
1046-5928
Keyword (Author)
Populus alba LS peroxidaseSinapyl alcoholRefoldingCell wall peroxidase
Keyword
CELL-WALL-PEROXIDASEESCHERICHIA-COLISYRINGALDAZINE OXIDASERECOMBINANT ENZYMELIGNINLIGNIFICATIONALCOHOLACTIVATIONISOENZYMECONIFERYL

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