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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 620 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 613 | - |
dc.citation.title | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.citation.volume | 19 | - |
dc.contributor.author | Choe, Hyunjun | - |
dc.contributor.author | Lee, Sumi | - |
dc.contributor.author | Hwang, Hyojin | - |
dc.contributor.author | Joo, Jeong Chan | - |
dc.contributor.author | Cho, Dae Haeng | - |
dc.contributor.author | Kim, Yong Hwan | - |
dc.date.accessioned | 2023-12-22T02:36:28Z | - |
dc.date.available | 2023-12-22T02:36:28Z | - |
dc.date.created | 2016-09-06 | - |
dc.date.issued | 2014-07 | - |
dc.description.abstract | The efficient regeneration of nicotinamide cofactors is an important process for industrial applications because of their high cost and stoichiometric requirements. In this study, the FDH1 beta-subunit of NAD-dependent formate dehydrogenase from Methylobacterium extorquens AM1 was heterologously expressed in Escherichia coli. It showed water-forming NADH oxidase (NOX-2) activity in the absence of its alpha-subunit. The beta-subunit oxidized NADH and generated NAD(+). The enzyme showed a low NADH oxidation activity (0.28 U/mg enzyme). To accelerate electron transfer from the enzyme to oxygen, four electron mediators were tested; flavin mononucleotide, flavin adenine dinucleotide, benzyl viologen (BV), and methyl viologen. All tested electron mediators increased enzyme activity; addition of 250 mu M BV resulted in the largest increase in enzyme activity (9.98 U/mg enzyme; a 35.6-fold increase compared with that in the absence of an electron mediator). Without the aid of an electron mediator, the enzyme had a substrate-binding affinity for NADH (K (m)) of 5.87 mu M, a turnover rate (k (cat)) of 0.24/sec, and a catalytic efficiency (k (cat)/K (m)) of 41.31/mM/sec. The addition of 50 mu M BV resulted in a 22.75-fold higher turnover rate (k (cat), 5.46/sec) and a 2.64-fold higher catalytic efficiency (k (cat)/K (m), 107.75/mM/sec) | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.19, no.4, pp.613 - 620 | - |
dc.identifier.doi | 10.1007/s12257-014-0126-1 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.scopusid | 2-s2.0-84907246671 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/20343 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs12257-014-0126-1 | - |
dc.identifier.wosid | 000342206100006 | - |
dc.language | 영어 | - |
dc.publisher | KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING | - |
dc.title | Expression of the NAD-dependent FDH1 beta-subunit from Methylobacterium extorquens AM1 in Escherichia coli and its characterization | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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