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Kim, Yong Hwan
Enzyme and Protein Engineering Lab.
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dc.citation.endPage 2748 -
dc.citation.number 6 -
dc.citation.startPage 2737 -
dc.citation.volume 100 - Baek, Seung-Ho - Kwon, Eunice Y. - Kim, Yong Hwan - Hahn, Ji-Sook - 2023-12-22T00:07:07Z - 2023-12-22T00:07:07Z - 2016-09-06 - 2016-03 -
dc.description.abstract There is an increasing demand for microbial production of lactic acid (LA) as a monomer of biodegradable poly lactic acid (PLA). Both optical isomers, D-LA and L-LA, are required to produce stereocomplex PLA with improved properties. In this study, we developed Saccharomyces cerevisiae strains for efficient production of D-LA. D-LA production was achieved by expressing highly stereospecific D-lactate dehydrogenase gene (ldhA, LEUM_1756) from Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 in S. cerevisiae lacking natural LA production activity. D-LA consumption after glucose depletion was inhibited by deleting DLD1 encoding D-lactate dehydrogenase and JEN1 encoding monocarboxylate transporter. In addition, ethanol production was reduced by deleting PDC1 and ADH1 genes encoding major pyruvate decarboxylase and alcohol dehydrogenase, respectively, and glycerol production was eliminated by deleting GPD1 and GPD2 genes encoding glycerol-3-phosphate dehydrogenase. LA tolerance of the engineered D-LA-producing strain was enhanced by adaptive evolution and overexpression of HAA1 encoding a transcriptional activator involved in weak acid stress response, resulting in effective D-LA production up to 48.9 g/L without neutralization. In a flask fed-batch fermentation under neutralizing condition, our evolved strain produced 112.0 g/L D-LA with a yield of 0.80 g/g glucose and a productivity of 2.2 g/(L center dot h) -
dc.identifier.bibliographicCitation APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.100, no.6, pp.2737 - 2748 -
dc.identifier.doi 10.1007/s00253-015-7174-0 -
dc.identifier.issn 0175-7598 -
dc.identifier.scopusid 2-s2.0-84959138098 -
dc.identifier.uri -
dc.identifier.url -
dc.identifier.wosid 000371244300020 -
dc.language 영어 -
dc.publisher SPRINGER -
dc.title Metabolic engineering and adaptive evolution for efficient production of D-lactic acid in Saccharomyces cerevisiae -
dc.type Article -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordAuthor Adaptive evolution -
dc.subject.keywordAuthor Acid tolerance -
dc.subject.keywordAuthor D-lactic acid -
dc.subject.keywordAuthor Metabolic engineering -
dc.subject.keywordAuthor Saccharomyces cerevisiae -
dc.subject.keywordPlus LACTATE-DEHYDROGENASE GENE -
dc.subject.keywordPlus ACETIC-ACID -
dc.subject.keywordPlus YEAST -
dc.subject.keywordPlus EXPRESSION -
dc.subject.keywordPlus TOLERANCE -
dc.subject.keywordPlus ETHANOL -
dc.subject.keywordPlus PHOSPHORYLATION -
dc.subject.keywordPlus CULTURES -
dc.subject.keywordPlus VECTORS -
dc.subject.keywordPlus STRESS -


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