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강주헌

Kang, Joo H.
Translational Multiscale Biofluidics Lab.
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dc.citation.endPage 11298 -
dc.citation.number 31 -
dc.citation.startPage 11293 -
dc.citation.title PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA -
dc.citation.volume 111 -
dc.contributor.author Shemesh, Jonathan -
dc.contributor.author Ben Arye, Tom -
dc.contributor.author Avesar, Jonathan -
dc.contributor.author Kang, Joo H. -
dc.contributor.author Fine, Amir -
dc.contributor.author Super, Michael -
dc.contributor.author Meller, Amit -
dc.contributor.author Ingber, Donald E. -
dc.contributor.author Levenberg, Shulamit -
dc.date.accessioned 2023-12-22T02:15:03Z -
dc.date.available 2023-12-22T02:15:03Z -
dc.date.created 2016-06-03 -
dc.date.issued 2014-08 -
dc.description.abstract Microfluidic water-in-oil droplets that serve as separate, chemically isolated compartments can be applied for single-cell analysis; however, to investigate encapsulated cells effectively over prolonged time periods, an array of droplets must remain stationary on a versatile substrate for optimal cell compatibility. We present here a platform of unique geometry and substrate versatility that generates a stationary nanodroplet array by using wells branching off a main microfluidic channel. These droplets are confined by multiple sides of a nanowell and are in direct contact with a biocompatible substrate of choice. The device is operated by a unique and reversed loading procedure that eliminates the need for fine pressure control or external tubing. Fluorocarbon oil isolates the droplets and provides soluble oxygen for the cells. By using this approach, the metabolic activity of single adherent cells was monitored continuously over time, and the concentration of viable pathogens in blood-derived samples was determined directly by measuring the number of colony-formed droplets. The method is simple to operate, requires a few microliters of reagent volume, is portable, is reusable, and allows for cell retrieval. This technology may be particularly useful for multiplexed assays for which prolonged and simultaneous visual inspection of many isolated single adherent or nonadherent cells is required. -
dc.identifier.bibliographicCitation PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.111, no.31, pp.11293 - 11298 -
dc.identifier.doi 10.1073/pnas.1404472111 -
dc.identifier.issn 0027-8424 -
dc.identifier.scopusid 2-s2.0-84905652885 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/19505 -
dc.identifier.url http://www.pnas.org/content/111/31/11293 -
dc.identifier.wosid 000339807200023 -
dc.language 영어 -
dc.publisher NATL ACAD SCIENCES -
dc.title Stationary nanoliter droplet array with a substrate of choice for single adherent/nonadherent cell incubation and analysis -
dc.type Article -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -

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