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Kang, Joo H.
Translational Multiscale Biofluidics Lab (TMB Lab)
Research Interests
  • Biomedical devices, infectious disease, organ-on-a-chip, microfluidics, mechanobiology

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Stationary nanoliter droplet array with a substrate of choice for single adherent/nonadherent cell incubation and analysis

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Title
Stationary nanoliter droplet array with a substrate of choice for single adherent/nonadherent cell incubation and analysis
Author
Shemesh, JonathanBen Arye, TomAvesar, JonathanKang, Joo H.Fine, AmirSuper, MichaelMeller, AmitIngber, Donald E.Levenberg, Shulamit
Keywords
single cell; nanoliter array; diagnostics
Issue Date
2014-08
Publisher
NATL ACAD SCIENCES
Citation
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.111, no.31, pp.11293 - 11298
Abstract
Microfluidic water-in-oil droplets that serve as separate, chemically isolated compartments can be applied for single-cell analysis; however, to investigate encapsulated cells effectively over prolonged time periods, an array of droplets must remain stationary on a versatile substrate for optimal cell compatibility. We present here a platform of unique geometry and substrate versatility that generates a stationary nanodroplet array by using wells branching off a main microfluidic channel. These droplets are confined by multiple sides of a nanowell and are in direct contact with a biocompatible substrate of choice. The device is operated by a unique and reversed loading procedure that eliminates the need for fine pressure control or external tubing. Fluorocarbon oil isolates the droplets and provides soluble oxygen for the cells. By using this approach, the metabolic activity of single adherent cells was monitored continuously over time, and the concentration of viable pathogens in blood-derived samples was determined directly by measuring the number of colony-formed droplets. The method is simple to operate, requires a few microliters of reagent volume, is portable, is reusable, and allows for cell retrieval. This technology may be particularly useful for multiplexed assays for which prolonged and simultaneous visual inspection of many isolated single adherent or nonadherent cells is required.
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DOI
10.1073/pnas.1404472111
ISSN
0027-8424
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BME_Journal Papers
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