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정웅규

Jung, Woonggyu
Translational Biophotonics Lab.
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One-photon and two-photon stimulation of neurons in a microfluidic culture system

Author(s)
Jang, Jae MyungLee, JeonghyeonKim, HyeongeunJeon, Noo LiJung, Woonggyu
Issued Date
2016-03
DOI
10.1039/C6LC00065G
URI
https://scholarworks.unist.ac.kr/handle/201301/18911
Fulltext
http://pubs.rsc.org/en/Content/ArticleLanding/2016/LC/C6LC00065G#!divAbstract
Citation
LAB ON A CHIP, v.16, no.9, pp.1684 - 1690
Abstract
In this paper, we demonstrate a novel platform for optical stimulation of neural circuits combined with microfluidic culture method and microelectrode-arrays measurement. Neuron-on-a-chip was designed and fabricated to isolate axons without a soma or dendrite. Thus, it is readily able to manipulate the neuronal alignment and to investigate the neuronal activity at the locations we want to observe. We adapted the optical stimulation technique to the arranged neurons to generate the neuronal signals in a non-invasive fashion. A blue light emitting diode and a femtosecond laser with 780 nm center wavelength were used for neuronal activation, and the corresponding neuronal signals were measured by MEAs at the same time. We found that one-photon light via caged glutamate provoked periodic spiking. In contrast the femtosecond pulse irradiation generated the repetitive firing at constant rates. Response times of one-photon and two-photon stimulation were around 200 ms and 50 ms, respectively. We also quantified neural responses, by varying optical parameters such as exposure time and irradiation power.
Publisher
ROYAL SOC CHEMISTRY
ISSN
1473-0197
Keyword
CHANNELSELECTRODENETWORKSPLATFORMCORTEX

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