Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.startPage | 20708 | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 6 | - |
dc.contributor.author | Lee, Hyunah | - |
dc.contributor.author | Nam, Donggyu | - |
dc.contributor.author | Choi, Jae Kyung | - |
dc.contributor.author | Araúzo‐Bravo, Marcos J | - |
dc.contributor.author | Kwon, Soon-Yong | - |
dc.contributor.author | Zaehres, Holm | - |
dc.contributor.author | Lee, Taehee | - |
dc.contributor.author | Park, Chan Young | - |
dc.contributor.author | Kang, Hyun Wook | - |
dc.contributor.author | Hans Robert Shoeler | - |
dc.contributor.author | Kim, Jeong Beom | - |
dc.date.accessioned | 2023-12-22T00:10:29Z | - |
dc.date.available | 2023-12-22T00:10:29Z | - |
dc.date.created | 2016-02-15 | - |
dc.date.issued | 2016-02 | - |
dc.description.abstract | The maintenance of undifferentiated human pluripotent stem cells (hPSC) under xeno-free condition requires the use of human feeder cells or extracellular matrix (ECM) coating. However, human-derived sources may cause human pathogen contamination by viral or non-viral agents to the patients. Here we demonstrate feeder-free and xeno-free culture system for hPSC expansion using diffusion assisted synthesis-grown nanocrystalline graphene (DAS-NG), a synthetic non-biological nanomaterial which completely rule out the concern of human pathogen contamination. DAS-NG exhibited advanced biocompatibilities including surface nanoroughness, oxygen containing functional groups and hydrophilicity. hPSC cultured on DAS-NG could maintain pluripotency in vitro and in vivo, and especially cell adhesion-related gene expression profile was comparable to those of cultured on feeders, while hPSC cultured without DAS-NG differentiated spontaneously with high expression of somatic cell-enriched adhesion genes. This feeder-free and xeno-free culture method using DAS-NG will facilitate the generation of clinical-grade hPSC. | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.6, pp.20708 | - |
dc.identifier.doi | 10.1038/srep20708 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.scopusid | 2-s2.0-84957564655 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/18576 | - |
dc.identifier.url | http://www.nature.com/articles/srep20708 | - |
dc.identifier.wosid | 000369542300001 | - |
dc.language | 영어 | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Establishment of feeder-free culture system for human induced pluripotent stem cell on DAS nanocrystalline graphene | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | SELF-RENEWAL | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | FILMS | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | LINES | - |
dc.subject.keywordPlus | SURFACES | - |
dc.subject.keywordPlus | PLATFORM | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.