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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 708 | - |
dc.citation.startPage | 701 | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 79 | - |
dc.contributor.author | Kim, Hyun Ju | - |
dc.contributor.author | Lim, Ji Won | - |
dc.contributor.author | Jeong, Haeyoung | - |
dc.contributor.author | Lee, Sang-Jae | - |
dc.contributor.author | Lee, Dong-Woo | - |
dc.contributor.author | Kim, Taesung | - |
dc.contributor.author | Lee, Sang Joon | - |
dc.date.accessioned | 2023-12-21T23:46:32Z | - |
dc.date.available | 2023-12-21T23:46:32Z | - |
dc.date.created | 2016-01-11 | - |
dc.date.issued | 2016-05 | - |
dc.description.abstract | Multiple copies of a cadC homolog encoding a heavy metal-responsive transcription factor were found in the genome of a bacterium isolated from ocean sediment, and the heavy metal responses of the encoded proteins were characterized using a fluorescence reporter assay. Each CadC regulator exhibited distinct specificity in response to heavy metal ions, indicating their potential use as modular heavy metal biosensors. Next, we constructed CadC-controlled T7 RNA transcription systems for intracellular signal amplification, i.e., higher sensitivity. Flow cytometry revealed that cadmium and lead ions could be recognized specifically by CadC-T7 biosensors, which could be combined with a microfluidic platform to generate heavy metal biosensor devices with increased sensitivity. Our results demonstrate the successful development of synthetic CadC-T7 genetic circuitry for use in improved heavy metal biosensor microfluidic devices. | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.79, pp.701 - 708 | - |
dc.identifier.doi | 10.1016/j.bios.2015.12.101 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.scopusid | 2-s2.0-84953312664 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/18089 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0956566315307545 | - |
dc.identifier.wosid | 000370309200096 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | Development of a highly specific and sensitive cadmium and lead microbial biosensor using synthetic CadC-T7 genetic circuitry | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology | - |
dc.relation.journalResearchArea | Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | CadC | - |
dc.subject.keywordAuthor | Heavy metal ions | - |
dc.subject.keywordAuthor | Microbial biosensor | - |
dc.subject.keywordAuthor | Microfluidic device | - |
dc.subject.keywordAuthor | T7 RNA polymerase | - |
dc.subject.keywordPlus | CELL | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | SENSOR | - |
dc.subject.keywordPlus | ZN(II) | - |
dc.subject.keywordPlus | SYSTEM | - |
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