Development of a highly specific and sensitive cadmium and lead microbial biosensor using synthetic CadC-T7 genetic circuitry
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- Title
- Development of a highly specific and sensitive cadmium and lead microbial biosensor using synthetic CadC-T7 genetic circuitry
- Author
- Kim, Hyun Ju; Lim, Ji Won; Jeong, Haeyoung; Lee, Sang-Jae; Lee, Dong-Woo; Kim, Taesung; Lee, Sang Joon
- Issue Date
- 2016-05
- Publisher
- ELSEVIER ADVANCED TECHNOLOGY
- Citation
- BIOSENSORS & BIOELECTRONICS, v.79, pp.701 - 708
- Abstract
- Multiple copies of a cadC homolog encoding a heavy metal-responsive transcription factor were found in the genome of a bacterium isolated from ocean sediment, and the heavy metal responses of the encoded proteins were characterized using a fluorescence reporter assay. Each CadC regulator exhibited distinct specificity in response to heavy metal ions, indicating their potential use as modular heavy metal biosensors. Next, we constructed CadC-controlled T7 RNA transcription systems for intracellular signal amplification, i.e., higher sensitivity. Flow cytometry revealed that cadmium and lead ions could be recognized specifically by CadC-T7 biosensors, which could be combined with a microfluidic platform to generate heavy metal biosensor devices with increased sensitivity. Our results demonstrate the successful development of synthetic CadC-T7 genetic circuitry for use in improved heavy metal biosensor microfluidic devices.
- URI
- https://scholarworks.unist.ac.kr/handle/201301/18089
- URL
- http://www.sciencedirect.com/science/article/pii/S0956566315307545
- DOI
- 10.1016/j.bios.2015.12.101
- ISSN
- 0956-5663
- Appears in Collections:
- MEN_Journal Papers
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