Chattering during the milling process causes severe problems on both the workpiece and cutting tools. However, chatter vibration is the inevitable phenomenon that operators require the prediction before the process or monitoring system to avoid the chatter in real-time. To predict the chatter vibration with the stability lobe diagram, the dynamic parameters of machine tool are extracted by considering cutting conditions and adapting the material properties. In this study, experimental verifications were taken for various aluminum types with different feed rates to observe the effect of the key parameters. The comparison between experimental results and the predictions was also performed.