BROWSE

Related Researcher

Author's Photo

Mitchell, Robert J.
Applied & Environmental Microbiology Lab (AEML)
Research Interests
  • Drug-Resistant Pathogens, Bdellovibrio bacteriovorus, patho-biotechnology

ITEM VIEW & DOWNLOAD

Effects of Carbon Dioxide Aerosols on the Viability of Escherichia coli during Biofilm Dispersal

DC Field Value Language
dc.contributor.author Singh, Renu ko
dc.contributor.author Monnappa, Ajay ko
dc.contributor.author Hong, Seongkyeol ko
dc.contributor.author Mitchell, Robert J. ko
dc.contributor.author Jang, Jaesung ko
dc.date.available 2015-09-16T02:57:25Z -
dc.date.created 2015-08-17 ko
dc.date.issued 2015-09 ko
dc.identifier.citation SCIENTIFIC REPORTS, v.5, pp.13766 ko
dc.identifier.issn 2045-2322 ko
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/16864 -
dc.description.abstract A periodic jet of carbon dioxide (CO2) aerosols is a very quick and effective mechanical technique to remove biofilms from various substrate surfaces. However, the impact of the aerosols on the viability of bacteria during treatment has never been evaluated. In this study, the effects of high-speed CO2 aerosols, a mixture of solid and gaseous CO2, on bacteria viability was studied. It was found that when CO2 aerosols were used to disperse biofilms of Escherichia coli, they led to a significant loss of viability, with approximately 50% of the dispersed bacteria killed in the process. By comparison, 75.6% of the biofilm-associated bacteria were viable when gently dispersed using Proteinase K and DNase I. Indirect proof that the aerosols are damaging the bacteria was found using a recombinant E. coli expressing the cyan fluorescent protein, as nearly half of the fluorescence was found in the supernatant after CO2 aerosol treatment, while the rest was associated with the bacterial pellet. In comparison, the supernatant fluorescence was only 9% when the enzymes were used to disperse the biofilm. As such, these CO2 aerosols not only remove biofilm-associated bacteria effectively but also significantly impact their viability by disrupting membrane integrity. ko
dc.description.statementofresponsibility open -
dc.language 영어 ko
dc.publisher NATURE PUBLISHING GROUP ko
dc.title Effects of Carbon Dioxide Aerosols on the Viability of Escherichia coli during Biofilm Dispersal ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-84941049046 ko
dc.identifier.wosid 000360798000001 ko
dc.type.rims ART ko
dc.description.wostc 0 *
dc.date.tcdate 2016-02-17 *
dc.identifier.doi 10.1038/srep13766 ko
dc.identifier.url http://www.nature.com/articles/srep13766 ko
Appears in Collections:
BIO_Journal Papers
MEN_Journal Papers

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show simple item record

qrcode

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU