O-GlcNAc cycling enzymes control vascular development of the placenta by modulating the levels of HIF-1α
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- O-GlcNAc cycling enzymes control vascular development of the placenta by modulating the levels of HIF-1α
- Yang, Yong Ryoul; Jang, Hyun-Jun; Lee, Yong Hwa; Kim, Il Shin; Lee, Ho; Ryu, Sung Ho; Suh, Pann-Ghill
- Issue Date
- W B SAUNDERS CO LTD
- PLACENTA, v.36, pp.1063 - 1068
- Introduction: Placental vasculogenesis is essential for fetal growth and development, and is affected profoundly by oxygen tension (hypoxia). Hypoxia-inducible factor-1 alpha: (HIF-1 alpha), which is stabilized at the protein level in response to hypoxia, is essential for vascular morphogenesis in the placenta. Many studies suggested that responses to hypoxia is influenced by O-GlcNAcylation. O-GlcNAcylation is regulated by O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) that catalyze the addition and removal of O-GlcNAc respectively.
Methods: We generated OGA deficient mice and evaluated OGA(-/-) placentas. The analysis of OGA(-/-) placentas was focused on morphological change and placental vasculogenesis. HIF-1 alpha: protein stability or transcriptional activity under dysregulation of O-GlcNAcylation were evaluated by Western blot, RT-qPCR and luciferase reporter gene assays in MEFs or MS1 cell line.
Results: Deletion of OGA results in defective placental vasculogenesis. OGA(-/-) placentas showed an abnormal placental shape and reduced vasculature in the labyrinth, which caused a developmental delay in the embryos. OGA deletion, which elevates O-GlcNAcylation and downregulates O-GlcNAc transferase (OGT), suppressed HIF-1 alpha stabilization and the transcription of its target genes. In contrast, the overexpression of O-GlcNAc cycling enzymes enhanced the expression and transcriptional activity of HIF-1 alpha.
Discussion: These results suggest that OGA plays a critical role in placental vasculogenesis by modulating HIP-1 alpha stabilization. Control of O-GlcNAcylation is essential for placental development. (C) 2015 Elsevier Ltd. All rights reserved.
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