There are no files associated with this item.
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 148 | - |
dc.citation.startPage | 141 | - |
dc.citation.title | METABOLIC ENGINEERING | - |
dc.citation.volume | 30 | - |
dc.contributor.author | Kim, Suk Min | - |
dc.contributor.author | Choi, Bae Young | - |
dc.contributor.author | Ryu, Young Shin | - |
dc.contributor.author | Jung, Sung Hun | - |
dc.contributor.author | Park, Jung Min | - |
dc.contributor.author | Kim, Goo-Hee | - |
dc.contributor.author | Lee, Sung Kuk | - |
dc.date.accessioned | 2023-12-22T00:38:09Z | - |
dc.date.available | 2023-12-22T00:38:09Z | - |
dc.date.created | 2015-09-02 | - |
dc.date.issued | 2015-11 | - |
dc.description.abstract | After glucose, xylose is the most abundant sugar in lignocellulosic carbon sources. However, wild-type Escherichia cob is unable to simultaneously utilize both sugars due to carbon catabolite repression (CCR). In this paper, we describe GX50, an engineered strain capable of utilizing glucose and xylose simultaneously. This strain was obtained by evolving a mutant from which araC has been deleted, and in which genes required for pentose metabolism are constitutively expressed. The strain acquired four additional mutations during adaptive evolution, including intergenic mutations in the 5'-flanking region of xylA and pyrE, and missense mutations in araE (S911) and ybjG (D99G). In contrast to wild type E. coli, GX50 rapidly converts xylose to xylitol even if glucose is available. Notably, the strain grows well when cultured on glucose, unlike some well-known CCR-insensitive mutants defective in the glucose phosphotransferase system. Our work will advance efforts to design a metabolically efficient platform strain for potential use in producing chemicals from lignocellulose. (C) 2015 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved | - |
dc.identifier.bibliographicCitation | METABOLIC ENGINEERING, v.30, pp.141 - 148 | - |
dc.identifier.doi | 10.1016/j.ymben.2015.05.002 | - |
dc.identifier.issn | 1096-7176 | - |
dc.identifier.scopusid | 2-s2.0-84931264120 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/16545 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S1096717615000646# | - |
dc.identifier.wosid | 000358424800015 | - |
dc.language | 영어 | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Simultaneous utilization of glucose and xylose via novel mechanisms in engineered Escherichia coli | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Lignocellulose | - |
dc.subject.keywordAuthor | Xylose | - |
dc.subject.keywordAuthor | Glucose | - |
dc.subject.keywordAuthor | Metabolic engineering | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.subject.keywordPlus | CATABOLITE REPRESSION | - |
dc.subject.keywordPlus | XYLITOL PRODUCTION | - |
dc.subject.keywordPlus | PHOSPHOTRANSFERASE SYSTEM | - |
dc.subject.keywordPlus | ADAPTIVE EVOLUTION | - |
dc.subject.keywordPlus | SUGAR MIXTURES | - |
dc.subject.keywordPlus | K-12 | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | GENOME | - |
dc.subject.keywordPlus | FERMENTATION | - |
dc.subject.keywordPlus | SEQUENCE | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.