BROWSE

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Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

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PHOSPHOLIPASE-C ASSOCIATED WITH PARTICULATE FRACTIONS OF BOVINE BRAIN

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dc.contributor.author Lee, Kee-Young ko
dc.contributor.author Ryu, Sung Ho ko
dc.contributor.author Suh, Pann-Ghill ko
dc.contributor.author Choi, Won Chul ko
dc.contributor.author Rhee, Sue Goo ko
dc.date.available 2015-09-01T08:03:52Z -
dc.date.created 2015-08-19 ko
dc.date.issued 1987-08 ko
dc.identifier.citation PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.84, no.16, pp.5540 - 5544 ko
dc.identifier.issn 0027-8424 ko
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/16514 -
dc.description.abstract We previously reported that cytosolic fractions of bovine brain contain two immunologically distinct phosphoinositide-specific phospholipases C (PLCs), PLC-I and PLC-II. In this report the subcellular distribution of PLC-I and PLC-II in brain homogenates was measured using RIA. Significant differences were found in the distribution of the two forms of PLC in 100,000 X g supernatants (cytosolic fraction) of brain homogenized in hypotonic buffer and 2 M KCl extracts of washed pellets (particulate fraction). More than 90% of PLC-II was found in the cytosolic fractions, whereas the PLC-I-like molecules were equally distributed between cytosolic and particulate fractions. Purification of PLC enzyme to near homogeneity from the particulate fractions yielded two PLC enzymes, both of which could be recognized by anti-PLC-I antibodies but not by anti-PLC-II antibodies. Their Mr values, determined under denaturing conditions, were 150,000 and 140,000. The polypeptide of the enzyme of Mr 150,000 seems to be the same as that of the cytosolic enzyme PLC-I: their Mr values were identical, and their trypsin-digested peptides yielded a similar elution profile on a C18 reverse-phase column. We propose, therefore, that PLC-I and its truncated form are weakly associated with membranes. ko
dc.description.statementofresponsibility close -
dc.language 영어 ko
dc.publisher NATL ACAD SCIENCES ko
dc.title PHOSPHOLIPASE-C ASSOCIATED WITH PARTICULATE FRACTIONS OF BOVINE BRAIN ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-0023388358 ko
dc.identifier.wosid A1987J650800007 ko
dc.type.rims ART ko
dc.description.wostc 115 *
dc.description.scopustc 48 *
dc.date.tcdate 2015-12-28 *
dc.date.scptcdate 2015-11-04 *
dc.identifier.doi 10.1073/pnas.84.16.5540 ko
dc.identifier.url http://www.pnas.org/content/84/16/5540?tab=author-info ko
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