There are no files associated with this item.
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 289 | - |
dc.citation.startPage | 283 | - |
dc.citation.title | BIOCHEMICAL JOURNAL | - |
dc.citation.volume | 331 | - |
dc.contributor.author | Park, Eu-Suk | - |
dc.contributor.author | Won, Jong Hyun | - |
dc.contributor.author | Han, Kee Jung | - |
dc.contributor.author | Suh, Pann-Ghill | - |
dc.contributor.author | Ryu, Sung Ho | - |
dc.contributor.author | Lee, Hee Sung | - |
dc.contributor.author | Yun, Hye-Young | - |
dc.contributor.author | Kwon, Nyoun Soo | - |
dc.contributor.author | Baek, Kwang Jin | - |
dc.date.accessioned | 2023-12-22T12:36:17Z | - |
dc.date.available | 2023-12-22T12:36:17Z | - |
dc.date.created | 2015-08-19 | - |
dc.date.issued | 1998-04 | - |
dc.description.abstract | Although the oxytocin receptor modulates intracellular Ca2+ ion levels in myometrium, the identities of signal molecules have not been clearly clarified. Our previous studies on oxytocin receptor signalling demonstrated that 80 kDa G(h) alpha is a signal mediator [Baek, Kwon, Lee, Kim, Muralidhar and Im (1996) Biochem. J. 315, 739-744]. To elucidate the effector in the oxytocin receptor signalling pathway, we evaluated the oxytocin-mediated activation of phospholipase C (PLC) by using solubilized membranes from human myometrium and a three-component preparation containing the oxytocin receptor-G(h) alpha-PLC-delta 1 complex. PLC-delta 1 activity in the three-component preparation, as well as PLC activity in solubilized membranes, was increased by oxytocin in the presence of Ca2+ and activated G(h) alpha (GTP-bound G(h) alpha). Furthermore the stimulated PLC-delta 1 activity resulting from activation of G(h) alpha via the oxytocin receptor was significantly attenuated by the selective oxytocin antagonist desGlyNH(2)d(CH2)5[Tyr(Me)(2),Thr(4)] ornithine vasotocin or GDP. Consistent with these observations, co-immunoprecipitation and co-immunoadsorption of PLC-delta 1 in the three-component preparation by anti-G(h7)alpha antibody resulted in the PLC-delta 1 being tightly coupled to activated G(h) alpha on stimulation of the oxytocin receptor. These results indicate that PLC-delta 1 is the effector for G(h) alpha-mediated oxytocin receptor signalling | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL JOURNAL, v.331, pp.283 - 289 | - |
dc.identifier.doi | 10.1042/bj3310283 | - |
dc.identifier.issn | 0264-6021 | - |
dc.identifier.scopusid | 2-s2.0-0032038597 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/16488 | - |
dc.identifier.url | http://www.biochemj.org/content/331/1/283 | - |
dc.identifier.wosid | 000072972500037 | - |
dc.language | 영어 | - |
dc.publisher | PORTLAND PRESS LTD | - |
dc.title | Phospholipase C-delta 1 and oxytocin receptor signalling: evidence of its role as an effector | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.