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Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

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Phospholipase C-delta 1 and oxytocin receptor signalling: evidence of its role as an effector

Cited 57 times inthomson ciCited 19 times inthomson ci
Title
Phospholipase C-delta 1 and oxytocin receptor signalling: evidence of its role as an effector
Author
Park, Eu-SukWon, Jong HyunHan, Kee JungSuh, Pann-GhillRyu, Sung HoLee, Hee SungYun, Hye-YoungKwon, Nyoun SooBaek, Kwang Jin
Issue Date
1998-04
Publisher
PORTLAND PRESS LTD
Citation
BIOCHEMICAL JOURNAL, v.331, pp.283 - 289
Abstract
Although the oxytocin receptor modulates intracellular Ca2+ ion levels in myometrium, the identities of signal molecules have not been clearly clarified. Our previous studies on oxytocin receptor signalling demonstrated that 80 kDa G(h) alpha is a signal mediator [Baek, Kwon, Lee, Kim, Muralidhar and Im (1996) Biochem. J. 315, 739-744]. To elucidate the effector in the oxytocin receptor signalling pathway, we evaluated the oxytocin-mediated activation of phospholipase C (PLC) by using solubilized membranes from human myometrium and a three-component preparation containing the oxytocin receptor-G(h) alpha-PLC-delta 1 complex. PLC-delta 1 activity in the three-component preparation, as well as PLC activity in solubilized membranes, was increased by oxytocin in the presence of Ca2+ and activated G(h) alpha (GTP-bound G(h) alpha). Furthermore the stimulated PLC-delta 1 activity resulting from activation of G(h) alpha via the oxytocin receptor was significantly attenuated by the selective oxytocin antagonist desGlyNH(2)d(CH2)5[Tyr(Me)(2),Thr(4)] ornithine vasotocin or GDP. Consistent with these observations, co-immunoprecipitation and co-immunoadsorption of PLC-delta 1 in the three-component preparation by anti-G(h7)alpha antibody resulted in the PLC-delta 1 being tightly coupled to activated G(h) alpha on stimulation of the oxytocin receptor. These results indicate that PLC-delta 1 is the effector for G(h) alpha-mediated oxytocin receptor signalling
URI
https://scholarworks.unist.ac.kr/handle/201301/16488
URL
http://www.biochemj.org/content/331/1/283
DOI
10.1042/bj3310283
ISSN
0264-6021
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BIO_Journal Papers
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