Overexpression of phospholipase C beta-1 protects NIH3T3 cells from oxidative stress-induced cell death

Abstract

Oxidative stress has been implicated in a wide range of cellular damage which includes DNA oxidation, membrane lipid peroxidation, and apoptosis. In our study, we found that overexpression of PLC-beta 1 in NIH3T3 fibroblasts protected them from cell death occuring in response to oxidative stress. Cell death caused by treatment with prooxidant tert-butylhydroperoxide (TBH), H2O2, or CdCl2 was considerably suppressed in PLC-beta 1 overexpressed NIH/beta 1-14 cells in comparison to control NIH/neo cells. However, overexpression of PLC-beta 1 failed to protect the cells from toxicity by diamide or KCN. In addition, while accumulation of c-fos mRNA was observed within 30 min of TBH treatment in vector transfected NIH/neo cells, TBH-induced c-fos mRNA generation was completely suppressed in NIH/beta 1-14 cells, while that of c-jun and GAPDH was not affected. These findings suggest that PLC-beta 1 may play a role in process that can protect cells from oxidative stress-induced cell death. (C) 2000 Elsevier Science Inc. All rights reserved