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DC Field | Value | Language |
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dc.citation.endPage | 185 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 178 | - |
dc.citation.title | JOURNAL OF NEUROCHEMISTRY | - |
dc.citation.volume | 71 | - |
dc.contributor.author | Bae, Sun Sik | - |
dc.contributor.author | Lee, Young Han | - |
dc.contributor.author | Chang, Jong-Soo | - |
dc.contributor.author | Galadari, Sehamuddin H. | - |
dc.contributor.author | Kim, Yong Sik | - |
dc.contributor.author | Ryu, Sung Ho | - |
dc.contributor.author | Suh, Pann-Ghill | - |
dc.date.accessioned | 2023-12-22T12:36:00Z | - |
dc.date.available | 2023-12-22T12:36:00Z | - |
dc.date.created | 2015-08-20 | - |
dc.date.issued | 1998-07 | - |
dc.description.abstract | Phospholipase C gamma 1 (PLC-gamma 1) is phosphorylated on treatment of cells with nerve growth factor (NGF).To assess the role of PLC-gamma 1 in mediating the neuronal differentiation induced by NGF treatment, we established PC12 cells that overexpress whole PLC-gamma 1 (PLC-gamma 1PC12), the SH2-SH2-SH3 domain (PLC-gamma 1SH223PC12), SH2-SH2-deleted mutants (PLC-gamma 1 Delta SH22PC12), and SH3-deleted mutants (PLC-gamma 1 Delta SH3PC12). Overexpressed whole PLC-yl or the SH2-SH2-SH3 domain of PLC-gamma 1 stimulated cell growth and inhibited NGF-induced neurite outgrowth of PC12 cells. However, cells expressing PLC-yl lacking the SH2-SH2 domain or the SH3 domain had no effect on NGF-induced neuronal differentiation. Overexpression of intact PLC-gamma 1 resulted in a threefold increase in total inositol phosphate accumulation on treatment with NGF. However, overexpression of the SH2-SH2-SH3 domain of PLC-gamma 1 did not alter total inositol phosphate accumulation. To investigate whether the SH2-SH2-SH3 domain of PLC-gamma 1 can mediate the NGF-induced signal, tyrosine phosphorylation of the SH2-SH2-SH3 domain of PLC-gamma 1 on NGF treatment was examined. The SH2-SH2-SH3 domain of PLC-gamma 1 as well as intact PLC-gamma 1 could be tyrosine-phosphorylated on NGF treatment. These results indicate that the overexpressed SH2-SH2-SH3 domain of PLC-gamma 1 can block the differentiation of PC12 cells induced by NGF and that the inhibition appears not to be related to the lipase activity of PLC-gamma 1 but to the SH2-SH2-SH3 domain of PLC-gamma 1 | - |
dc.identifier.bibliographicCitation | JOURNAL OF NEUROCHEMISTRY, v.71, no.1, pp.178 - 185 | - |
dc.identifier.doi | 10.1046/j.1471-4159.1998.71010178.x | - |
dc.identifier.issn | 0022-3042 | - |
dc.identifier.scopusid | 2-s2.0-0346629933 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/16457 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1046/j.1471-4159.1998.71010178.x/abstract | - |
dc.identifier.wosid | 000074283600019 | - |
dc.language | 영어 | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | Src homology domains of phospholipase C gamma 1 inhibit nerve growth factor-induced differentiation of PC12 cells | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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