There are no files associated with this item.
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 5860 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 5852 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 279 | - |
dc.contributor.author | Hur, EM | - |
dc.contributor.author | Park, YS | - |
dc.contributor.author | Lee, BD | - |
dc.contributor.author | Jang, IH | - |
dc.contributor.author | Kim, HS | - |
dc.contributor.author | Kim, TD | - |
dc.contributor.author | Suh, Pann-Ghill | - |
dc.contributor.author | Ryu, SH | - |
dc.contributor.author | Kim, KT | - |
dc.date.accessioned | 2023-12-22T11:07:00Z | - |
dc.date.available | 2023-12-22T11:07:00Z | - |
dc.date.created | 2015-08-20 | - |
dc.date.issued | 2004-02 | - |
dc.description.abstract | Communication between receptor tyrosine kinase (RTK)- and G protein-coupled receptor (GPCR)- mediated signaling systems has received increasing attention in recent years. Here, we report that activation of G protein-coupled bradykinin B-2 receptor induces an up-regulation of cellular responses mediated by epidermal growth factor receptor (EGFR) and provide essential mechanistic characteristics of this sensitization process. EGF, which failed to evoke detectable amount of calcium increase and neurotransmitter release when administrated alone in primary cultures of rat adrenal chromaffin cells and PC12 cells, became capable of inducing these responses specifically after bradykinin pretreatment. Both EGFR and non-receptor tyrosine kinase p60(Src), whose kinase activities were required in the sensitization, were found to be enriched in cholesterolrich lipid rafts. Bradykinin caused activation of p60(Src) and Src-dependent phosphorylation of the EGFR on Tyr-845 in lipid rafts, as well as recruitment of phospholipase C (PLC) gamma1 to the rafts. Depletion of cholesterol by methyl-beta-cyclodextrin disrupted the raft localization of EGFR and Src, as well as bradykinin-induced translocation of PLCgamma1. Furthermore, sensitization, which was impaired by cholesterol depletion, was restored by repletion of cholesterol. Therefore, we suggest that lipid rafts are essential participants in the regulation of receptor-mediated signal transduction and cross-talk via organizing signaling complexes in membrane microdomains | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.279, no.7, pp.5852 - 5860 | - |
dc.identifier.doi | 10.1074/jbc.M311687200 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.scopusid | 2-s2.0-1242316984 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/16441 | - |
dc.identifier.wosid | 000188776500100 | - |
dc.language | 영어 | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.title | Sensitization of epidermal growth factor-induced signaling by bradykinin is mediated by c-Src - Implications for a role of lipid microdomains | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.