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Kwon, Taejoon
TaejoonLab
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  • Systems biology, computational biology, functional genomics

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Id2a functions to limit Notch pathway activity and thereby influence the transition from proliferation to differentiation of retinoblasts during zebrafish retinogenesis

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dc.contributor.author Uribe, Rosa A. ko
dc.contributor.author Kwon, Taejoon ko
dc.contributor.author Marcotte, Edward M. ko
dc.contributor.author Gross, Jeffrey M. ko
dc.date.available 2015-08-05T00:25:54Z -
dc.date.created 2015-08-04 ko
dc.date.issued 2012-11 -
dc.identifier.citation DEVELOPMENTAL BIOLOGY, v.371, no.2, pp.280 - 292 ko
dc.identifier.issn 0012-1606 ko
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/13327 -
dc.identifier.uri http://www.sciencedirect.com/science/article/pii/S0012160612004915 ko
dc.description.abstract During vertebrate retinogenesis, the precise balance between retinoblast proliferation and differentiation is spatially and temporally regulated through a number of intrinsic factors and extrinsic signaling pathways. Moreover, there are complex gene regulatory network interactions between these intrinsic factors and extrinsic pathways, which ultimately function to determine when retinoblasts exit the cell cycle and terminally differentiate. We recently uncovered a cell non-autonomous role for the intrinsic HLH factor, Id2a, in regulating retinoblaslt proliferation and differentiation, with Id2a-deficient retinae containing an abundance of proliferative retinoblasts and an absence of terminally differentiated retinal neurons and glia. Here, we report that Id2a function is necessary and sufficient to limit Notch pathway activity during retinogenesis. Id2a-deficient retinae possess elevated levels of Notch pathway component gene expression, while retinae overexpressing id2a possess reduced expression of Notch pathway component genes. Attenuation of Notch, signaling activity by DAPT or by morpholino knockdown of Notch1a is sufficient to rescue both the proliferative and differentiation defects in Id2a-deficient retinae. In addition to regulating Notch pathway activity, through a novel RNA-Seq and differential gene expression analysis of Id2a-deficient retinae, we identify a number of additional intrinsic and extrinsic regulatory pathway components whose, expression is regulated by Id2a. These data highlight the integral role played by Id2a in the gene regulatory network governing the transition from retinoblast proliferation to terminal differentiation during vertebrate retinogenesis. (C) 2012 Elsevier Inc. All rights reserved ko
dc.description.statementofresponsibility close -
dc.language ENG ko
dc.publisher ACADEMIC PRESS INC ELSEVIER SCIENCE ko
dc.subject VISUAL-SYSTEM DEVELOPMENT ko
dc.subject LARGE GENE LISTS ko
dc.subject NEURONAL DIFFERENTIATION ko
dc.subject RETINAL DEVELOPMENT ko
dc.subject TRANSCRIPTION FACTORS ko
dc.subject VERTEBRATE RETINA ko
dc.subject PROGENITOR CELLS ko
dc.subject BHLH GENES ko
dc.subject DELTA-LIKE ko
dc.subject CYCLIN D1 ko
dc.title Id2a functions to limit Notch pathway activity and thereby influence the transition from proliferation to differentiation of retinoblasts during zebrafish retinogenesis ko
dc.title.alternative Id2a functions to limit Notch pathway activity and thereby influence the transition from proliferation to differentiation of retinoblasts during zebrafish retinogenesis ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-84867140313 ko
dc.identifier.wosid 000310101800015 ko
dc.type.rims ART ko
dc.description.wostc 8 *
dc.description.scopustc 4 *
dc.description.scopustc 4 *
dc.date.tcdate 2015-12-28 *
dc.date.scptcdate 2015-11-04 *
dc.date.scptcdate 2015-11-04 *
dc.identifier.doi 10.1016/j.ydbio.2012.08.032 ko
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