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DC Field | Value | Language |
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dc.citation.endPage | 480 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 474 | - |
dc.citation.title | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.citation.volume | 39 | - |
dc.contributor.author | Kim, Jungbae | - |
dc.contributor.author | Jia, Hongfei | - |
dc.contributor.author | Lee, Chang-won | - |
dc.contributor.author | Chung, Seung-wook | - |
dc.contributor.author | Kwak, Jahun | - |
dc.contributor.author | Shin, Yongsoon | - |
dc.contributor.author | Dohnalkova, Alice | - |
dc.contributor.author | Kim, Byung-Gee | - |
dc.contributor.author | Wang, Ping | - |
dc.contributor.author | Grate, Jay W. | - |
dc.date.accessioned | 2023-12-22T09:44:56Z | - |
dc.date.available | 2023-12-22T09:44:56Z | - |
dc.date.created | 2015-07-21 | - |
dc.date.issued | 2006-07 | - |
dc.description.abstract | Single enzyme nanoparticles of alpha-chymotrypsin (SEN-CT), in which each CT molecule is surrounded by a thin polymeric organic/inorganic network, stabilized the CT activity in a shaking condition as well as in a non-shaking condition. Since SEN-CT is soluble in a buffer solution and less than 10 nm in size, SEN-CT could be further immobilized in nanoporous silica with an average pore size of 29 nm. Free CT and SEN-CT were immobilized in nanoporous silica (NPS), and nanoporous silica that was first silanized with aminopropyltriethoxysilane (amino-NPS) to generate a positive surface charge. The SEN-CT adsorbed in amino-NPS was more stable than CT immobilized by either adsorption in NPS or covalent bonding to amino-NPS. In shaking conditions, nanoporous silica provided an additional stabilization by protecting SEN-CT from shear stresses. At 22 degrees C with harsh shaking, free, NPS-adsorbed and NPS-covalently attached CT showed half lives of 1, 62, and 80 h, respectively; whereas SEN-CT adsorbed in amino-NPS showed no activity loss within 12 days. The combination of SENs and nanoporous silica, which makes an active and stable immobilized enzyme system, represents a new structure for biocatalytic applications. (c) 2006 Elsevier Inc. All rights reserved | - |
dc.identifier.bibliographicCitation | ENZYME AND MICROBIAL TECHNOLOGY, v.39, no.3, pp.474 - 480 | - |
dc.identifier.doi | 10.1016/j.enzmictec.2005.11.042 | - |
dc.identifier.issn | 0141-0229 | - |
dc.identifier.scopusid | 2-s2.0-33646796717 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/12428 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0141022906001165# | - |
dc.identifier.wosid | 000238311900021 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.title.alternative | Single enzyme nanoparticles in nanoporous silica: A hierarchical approach to enzyme stabilization and immobilization | - |
dc.title | Single enzyme nanoparticles in nanoporous silica: A hierarchical approach to enzyme stabilization and immobilization | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | single enzyme nanoparticles | - |
dc.subject.keywordAuthor | nanoporous silica | - |
dc.subject.keywordAuthor | mesocellular foams | - |
dc.subject.keywordAuthor | alpha-chymotrypsin | - |
dc.subject.keywordAuthor | enzyme stabilization | - |
dc.subject.keywordAuthor | enzyme immobilization | - |
dc.subject.keywordPlus | MESOPOROUS SILICA | - |
dc.subject.keywordPlus | CATALYTIC ACTIVITY | - |
dc.subject.keywordPlus | ORGANIC-SOLVENTS | - |
dc.subject.keywordPlus | BIOCATALYSIS | - |
dc.subject.keywordPlus | CHYMOTRYPSIN | - |
dc.subject.keywordPlus | SUPPORT | - |
dc.subject.keywordPlus | SURFACE | - |
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