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Kwak, Ja Hun
Molecular Catalysis lab
Research Interests
  • Heterogeneous catalysis, molecular catalysis, ASlumima, zeolites

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Magnetically-separable and highly-stable enzyme system based on crosslinked enzyme aggregates shipped in magnetite-coated mesoporous silica

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dc.contributor.author Lee, Jinwoo ko
dc.contributor.author Na, Hyon Bin ko
dc.contributor.author Kim, Byoung Chan ko
dc.contributor.author Lee, Jin Hyung ko
dc.contributor.author Lee, Byoungsoo ko
dc.contributor.author Kwak, Jahun ko
dc.contributor.author Hwang, Yosun ko
dc.contributor.author Park, Je-Geun ko
dc.contributor.author Gu, Man Bock ko
dc.contributor.author Kim, Jaeyun ko
dc.contributor.author Joo, Jin ko
dc.contributor.author Shin, Chae-Ho ko
dc.contributor.author Grate, Jay W. ko
dc.contributor.author Hyeon, Taeghwan ko
dc.contributor.author Kim, Jungbae ko
dc.date.available 2015-07-23T02:12:01Z -
dc.date.created 2015-07-20 ko
dc.date.issued 2009-05 -
dc.identifier.citation JOURNAL OF MATERIALS CHEMISTRY, v.19, no.42, pp.7864 - 7870 ko
dc.identifier.issn 0959-9428 ko
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/12329 -
dc.identifier.uri http://pubs.rsc.org/en/Content/ArticleLanding/2009/JM/b909109b#!divAbstract ko
dc.description.abstract A magnetically-separable and highly-stable enzyme system was developed by adsorption of enzymes in superparamagnetic hierarchically ordered mesocellular mesoporous silica (M-HMMS) and subsequent enzyme crosslinking. Superparamagnetic nanoparticles were homogeneously incorporated into hierarchically-ordered mesocellular mesoporous silica (HMMS) by the decomposition of a preformed iron propionate complex. The size of the incorporated superparamagnetic nanoparticles was around 5 nm, generating a magnetically separable host with high pore volumes and large pores (M-HMMS). alpha-chymotrypsin (CT) was adsorbed into M-HMMS with high loading (similar to 30 wt%) in less than 30 minutes. Glutaraldehyde (GA) treatment of adsorbed CT resulted in nanometer scale crosslinked enzyme aggregates in M-HMMS (CLEA-M). The activity of these CT aggregates in M-HMMS (CLEA-M-CT) was 34 times than that of simply adsorbed CT in M-HMMS, due to an effective prevention of enzyme leaching during washing via a ship-in-a-bottle approach. CLEA-M-CT maintained the initial activity not only under shaking (250 rpm) for 30 days, but also under recycled uses of 35 times. The same approach was employed for the synthesis of CLEA-M of lipase (CLEA-M-LP), and proven to be effective in improving the loading, activity, and stability of enzyme when compared to those of adsorbed LP in M-HMMS ko
dc.description.statementofresponsibility close -
dc.language ENG ko
dc.publisher ROYAL SOC CHEMISTRY ko
dc.subject MESOCELLULAR CARBON FOAM ko
dc.subject A-BOTTLE SYNTHESIS ko
dc.subject POTENTIAL APPLICATIONS ko
dc.subject CATALYTIC ACTIVITY ko
dc.subject ORGANIC- ko
dc.title Magnetically-separable and highly-stable enzyme system based on crosslinked enzyme aggregates shipped in magnetite-coated mesoporous silica ko
dc.title.alternative Magnetically-separable and highly-stable enzyme system based on crosslinked enzyme aggregates shipped in magnetite-coated mesoporous silica ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-70350222069 ko
dc.identifier.wosid 000270942100009 ko
dc.type.rims ART ko
dc.description.wostc 25 *
dc.description.scopustc 27 *
dc.date.tcdate 2015-12-28 *
dc.date.scptcdate 2015-11-04 *
dc.identifier.doi 10.1039/b909109b ko
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