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Engineering Escherichia coli for Effective Expression of Plant Genes

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Title
Engineering Escherichia coli for Effective Expression of Plant Genes
Author
Lee, Junhyeong
Advisor
Lee, Sung Kuk
Keywords
Escherichia coli; Arabidopsis thaliana; heterologous gene expression; tRNA; chaperone
Issue Date
2015-02
Publisher
Graduate School of UNIST
Abstract
Various plant-derived useful chemicals have been used for human from ancient to modern time. Because of difference of genetic characters between bacteria and plant, it could cause low plant protein expression or formation of inclusion body composed by insoluble protein precipitates. There are generally two methods for enhancement of heterologous protein expression in E. coli. In order to change rare tRNA abundance and chaperone expression level, we conducted chromosomally integrated promoter mutant libraries using recombination with single-strand DNA oligonucleotides. Some chaperone promoter mutants showed enhancement of Arabidopsis’s protein expression level. The tRNA and chaperone promoter mutants also showed significant increases in target Arabidopsis’s protein expression. We developed E. coli mutants for optimal plant protein expression. It is applicable to pharmaceutical production of plant-derived useful chemicals from E. coli.
Description
Department Of Biomedical Engineering
URI
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