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DC Field | Value | Language |
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dc.citation.endPage | 5325 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 5315 | - |
dc.citation.title | JOURNAL OF PROTEOME RESEARCH | - |
dc.citation.volume | 10 | - |
dc.contributor.author | Yoon, Jong Hyuk | - |
dc.contributor.author | Song, Parkyong | - |
dc.contributor.author | Jang, Jin-Hyeok | - |
dc.contributor.author | Kim, Dae-Kyum | - |
dc.contributor.author | Choi, Sunkyu | - |
dc.contributor.author | Kim, Jaeyoon | - |
dc.contributor.author | Ghim, Jaewang | - |
dc.contributor.author | Kim, Dayea | - |
dc.contributor.author | Park, Sehoon | - |
dc.contributor.author | Lee, Hyeongji | - |
dc.contributor.author | Kwak, Dongoh | - |
dc.contributor.author | Yea, Kyungmoo | - |
dc.contributor.author | Hwang, Daehee | - |
dc.contributor.author | Suh, Pann-Ghill | - |
dc.contributor.author | Ryu, Sung Ho | - |
dc.date.accessioned | 2023-12-22T05:39:10Z | - |
dc.date.available | 2023-12-22T05:39:10Z | - |
dc.date.created | 2015-01-12 | - |
dc.date.issued | 2011-12 | - |
dc.description.abstract | There is a strong possibility that skeletal muscle can respond to irregular metabolic states by secreting specific cytokines. Obesity-related chronic inflammation, mediated by pro-inflammatory cytokines, is believed to be one of the causes of insulin resistance that results in type 2 diabetes. Here, we attempted to identify and characterize the members of the skeletal muscle secretome in response to tumor necrosis factor-alpha (TNF-alpha)-induced insulin resistance. To conduct this study, we comparatively analyzed the media levels of proteins released from L6 skeletal muscle cells. We found 28 TNF-a modulated secretory proteins by using separate filtering methods: Gene Ontology, SignalP, and SecretomeP, as well as the normalized Spectral Index for label-free quantification. Ten of these secretory proteins were increased and 18 secretory proteins were decreased by TNF-a treatment. Using microarray analysis of Zuker diabetic rat skeletal muscle combined with bioinformatics and QPCR, we found a correlation between TNF-alpha-mediated insulin resistance and type 2 diabetes. This novel approach combining analysis of the conditioned secretome and transcriptome has identified several previously unknown, TNF-alpha-dependent secretory proteins, which establish a foothold for research on the different causes of insulin resistance and their relationships with each other. | - |
dc.identifier.bibliographicCitation | JOURNAL OF PROTEOME RESEARCH, v.10, no.12, pp.5315 - 5325 | - |
dc.identifier.doi | 10.1021/pr200573b | - |
dc.identifier.issn | 1535-3893 | - |
dc.identifier.scopusid | 2-s2.0-82755184961 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/10093 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=82755184961 | - |
dc.identifier.wosid | 000297537200005 | - |
dc.language | 영어 | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Proteomic Analysis of Tumor Necrosis Factor-Alpha (TNF-alpha)-Induced L6 Myotube Secretome Reveals Novel TNF-alpha-Dependent Myokines in Diabetic Skeletal Muscle | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | proteomics | - |
dc.subject.keywordAuthor | insulin resistance | - |
dc.subject.keywordAuthor | chronic inflammation | - |
dc.subject.keywordAuthor | diabetes | - |
dc.subject.keywordAuthor | secretory proteins | - |
dc.subject.keywordAuthor | TNF-alpha | - |
dc.subject.keywordPlus | INSULIN-RESISTANCE | - |
dc.subject.keywordPlus | ADIPOSE-TISSUE | - |
dc.subject.keywordPlus | MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | CALORIC RESTRICTION | - |
dc.subject.keywordPlus | ANDROGEN RECEPTOR | - |
dc.subject.keywordPlus | APOLIPOPROTEIN-J | - |
dc.subject.keywordPlus | HUMAN OBESITY | - |
dc.subject.keywordPlus | TRANSCRIPTOME | - |
dc.subject.keywordPlus | CELLS | - |
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