Proteomic Analysis of Tumor Necrosis Factor-Alpha (TNF-alpha)-Induced L6 Myotube Secretome Reveals Novel TNF-alpha-Dependent Myokines in Diabetic Skeletal Muscle
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- Proteomic Analysis of Tumor Necrosis Factor-Alpha (TNF-alpha)-Induced L6 Myotube Secretome Reveals Novel TNF-alpha-Dependent Myokines in Diabetic Skeletal Muscle
- Yoon, Jong Hyuk; Song, Parkyong; Jang, Jin-Hyeok; Kim, Dae-Kyum; Choi, Sunkyu; Kim, Jaeyoon; Ghim, Jaewang; Kim, Dayea; Park, Sehoon; Lee, Hyeongji; Kwak, Dongoh; Yea, Kyungmoo; Hwang, Daehee; Suh, Pann-Ghill; Ryu, Sung Ho
- Issue Date
- AMER CHEMICAL SOC
- JOURNAL OF PROTEOME RESEARCH, v.10, no.12, pp.5315 - 5325
- There is a strong possibility that skeletal muscle can respond to irregular metabolic states by secreting specific cytokines. Obesity-related chronic inflammation, mediated by pro-inflammatory cytokines, is believed to be one of the causes of insulin resistance that results in type 2 diabetes. Here, we attempted to identify and characterize the members of the skeletal muscle secretome in response to tumor necrosis factor-alpha (TNF-alpha)-induced insulin resistance. To conduct this study, we comparatively analyzed the media levels of proteins released from L6 skeletal muscle cells. We found 28 TNF-a modulated secretory proteins by using separate filtering methods: Gene Ontology, SignalP, and SecretomeP, as well as the normalized Spectral Index for label-free quantification. Ten of these secretory proteins were increased and 18 secretory proteins were decreased by TNF-a treatment. Using microarray analysis of Zuker diabetic rat skeletal muscle combined with bioinformatics and QPCR, we found a correlation between TNF-alpha-mediated insulin resistance and type 2 diabetes. This novel approach combining analysis of the conditioned secretome and transcriptome has identified several previously unknown, TNF-alpha-dependent secretory proteins, which establish a foothold for research on the different causes of insulin resistance and their relationships with each other.
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