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이성국

Lee, Sung Kuk
Synthetic Biology & Metabolic Engineering Lab.
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dc.citation.number 4 -
dc.citation.startPage e94266 -
dc.citation.title PLOS ONE -
dc.citation.volume 9 -
dc.contributor.author Ryu, Young Shin -
dc.contributor.author Biswas, Rajesh Kumar -
dc.contributor.author Shin, Kwangsu -
dc.contributor.author Parisutham, Vinuselvi -
dc.contributor.author Kim, Suk Min -
dc.contributor.author Lee, Sung Kuk -
dc.date.accessioned 2023-12-22T02:43:24Z -
dc.date.available 2023-12-22T02:43:24Z -
dc.date.created 2015-01-12 -
dc.date.issued 2014-04 -
dc.description.abstract Multiplex genome engineering is a standalone recombineering tool for large-scale programming and accelerated evolution of cells. However, this advanced genome engineering technique has been limited to use in selected bacterial strains. We developed a simple and effective strain-independent method for effective genome engineering in Escherichia coli. The method involves introducing a suicide plasmid carrying the l Red recombination system into the mutS gene. The suicide plasmid can be excised from the chromosome via selection in the absence of antibiotics, thus allowing transient inactivation of the mismatch repair system during genome engineering. In addition, we developed another suicide plasmid that enables integration of large DNA fragments into the lacZ genomic locus. These features enable this system to be applied in the exploitation of the benefits of genome engineering in synthetic biology, as well as the metabolic engineering of different strains of E. coli. -
dc.identifier.bibliographicCitation PLOS ONE, v.9, no.4, pp.e94266 -
dc.identifier.doi 10.1371/journal.pone.0094266 -
dc.identifier.issn 1932-6203 -
dc.identifier.scopusid 2-s2.0-84899666828 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/10090 -
dc.identifier.wosid 000335226500021 -
dc.language 영어 -
dc.publisher PUBLIC LIBRARY SCIENCE -
dc.title A Simple and Effective Method for Construction of Escherichia coli Strains Proficient for Genome Engineering -
dc.type Article -
dc.description.isOpenAccess TRUE -
dc.relation.journalWebOfScienceCategory Multidisciplinary Sciences -
dc.relation.journalResearchArea Science & Technology - Other Topics -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -

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