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Ha, Taek Jip
The Ha Research Group
Research Interests
  • Single-Molecule Biophysics

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An improved surface passivation method for single-molecule studies

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Title
An improved surface passivation method for single-molecule studies
Author
Hua, BoyangHan, Kyu YoungZhou, RuoboKim, HajinShi, XinghuaAbeysirigunawardena, Sanjaya C.Jain, AnkurSingh, DigvijayAggarwal, VasudhaWoodson, Sarah A.Ha, Taek Jip
Keywords
IN-VITRO; PROTEINS; HELICASE; DNA; INITIATION; DYNAMICS; COMPLEX; MYOSIN
Issue Date
201412
Publisher
NATURE PUBLISHING GROUP
Citation
NATURE METHODS, v.11, no.12, pp.1233 - 1236
Abstract
We report a surface passivation method based on dichlorodimethylsilane (DDS)-Tween-20 for in vitro single-molecule studies, which, under the conditions tested here, more efficiently prevented nonspecific binding of biomolecules than the standard poly (ethylene glycol) surface. The DDS-Tween-20 surface was simple and inexpensive to prepare and did not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution.
URI
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DOI
http://dx.doi.org/10.1038/NMETH.3143
ISSN
1548-7091
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