An improved surface passivation method for single-molecule studies
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- An improved surface passivation method for single-molecule studies
- Hua, Boyang; Han, Kyu Young; Zhou, Ruobo; Kim, Hajin; Shi, Xinghua; Abeysirigunawardena, Sanjaya C.; Jain, Ankur; Singh, Digvijay; Aggarwal, Vasudha; Woodson, Sarah A.; Ha, Taek Jip
- IN-VITRO; PROTEINS; HELICASE; DNA; INITIATION; DYNAMICS; COMPLEX; MYOSIN
- Issue Date
- NATURE PUBLISHING GROUP
- NATURE METHODS, v.11, no.12, pp.1233 - 1236
- We report a surface passivation method based on dichlorodimethylsilane (DDS)-Tween-20 for in vitro single-molecule studies, which, under the conditions tested here, more efficiently prevented nonspecific binding of biomolecules than the standard poly (ethylene glycol) surface. The DDS-Tween-20 surface was simple and inexpensive to prepare and did not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution.
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