JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.130, no.12, pp.3726 - 3727
Abstract
A neutral tripod system for biotin-avidin assays, which is composed of biotin, FIrpic as an energy acceptor, and mCP as an energy donor, offers remarkable sensitivity over traditional transition metal based protein probes due to the intramolecular energy transfer and increased hydrophobicity associated with the avidin binding site and neutral probe 1.