Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.number | 1 | - |
dc.citation.startPage | 2732 | - |
dc.citation.title | NATURE COMMUNICATIONS | - |
dc.citation.volume | 15 | - |
dc.contributor.author | Kim, Suk Min | - |
dc.contributor.author | Kang, Sung Heuck | - |
dc.contributor.author | Lee, Jinhee | - |
dc.contributor.author | Heo, Yoonyoung Heo | - |
dc.contributor.author | Poloniataki, Eleni G. | - |
dc.contributor.author | Kang, Jingu | - |
dc.contributor.author | Yoon, Hye-Jin | - |
dc.contributor.author | Kong, So Yeon | - |
dc.contributor.author | Yun, Yaejin | - |
dc.contributor.author | Kim, Hyunwoo | - |
dc.contributor.author | Ryu, Jungki | - |
dc.contributor.author | Lee, Hyung Ho | - |
dc.contributor.author | Kim, Yong Hwan | - |
dc.date.accessioned | 2024-03-29T13:35:09Z | - |
dc.date.available | 2024-03-29T13:35:09Z | - |
dc.date.created | 2024-03-29 | - |
dc.date.issued | 2024-03 | - |
dc.description.abstract | Fe‒S cluster-harboring enzymes, such as carbon monoxide dehydrogenases (CODH), employ sophisticated artificial electron mediators like viologens to serve as potent biocatalysts capable of cleaning-up industrial off-gases at stunning reaction rates. Unraveling the interplay between these enzymes and their associated mediators is essential for improving the efficiency of CODHs. Here we show the electron mediator-interaction site on ChCODHs (Ch, Carboxydothermus hydrogenoformans) using a systematic approach that leverages the viologen-reactive characteristics of superficial aromatic residues. By enhancing mediator-interaction (R57G/N59L) near the D-cluster, the strategically tailored variants exhibit a ten-fold increase in ethyl viologen affinity relative to the wild-type without sacrificing the turn-over rate (kcat). Viologen-complexed structures reveal the pivotal positions of surface phenylalanine residues, serving as external conduits for the D-cluster to/from viologen. One variant (R57G/N59L/A559W) can treat a broad spectrum of waste gases (from steel-process and plastic-gasification) containing O2. Decoding mediator interactions will facilitate the development of industrially high-efficient biocatalysts encompassing gas-utilizing enzymes. | - |
dc.identifier.bibliographicCitation | NATURE COMMUNICATIONS, v.15, no.1, pp.2732 | - |
dc.identifier.doi | 10.1038/s41467-024-46909-1 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.scopusid | 2-s2.0-85188937578 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/81896 | - |
dc.identifier.wosid | 001195623200031 | - |
dc.language | 영어 | - |
dc.publisher | NATURE PORTFOLIO | - |
dc.title | Identifying a key spot for electron mediator interaction to tailor CO dehydrogenase’s affinity | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | CARBON-MONOXIDE DEHYDROGENASE | - |
dc.subject.keywordPlus | REDUCTION | - |
dc.subject.keywordPlus | ELECTROCHEMISTRY | - |
dc.subject.keywordPlus | CATALYSIS | - |
dc.subject.keywordPlus | FORMATE | - |
dc.subject.keywordPlus | SPACE | - |
dc.subject.keywordPlus | SITE | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.