Full metadata record
DC Field | Value | Language |
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dc.citation.title | BIOMATERIALS RESEARCH | - |
dc.citation.volume | 28 | - |
dc.contributor.author | Son, Boram | - |
dc.contributor.author | Park, Sora | - |
dc.contributor.author | Cho, Sungwoo | - |
dc.contributor.author | Kim, Jeong Ah | - |
dc.contributor.author | Baek, Seung-Ho | - |
dc.contributor.author | Yoo, Ki Hyun | - |
dc.contributor.author | Han, Dongoh | - |
dc.contributor.author | Joo, Jinmyoung | - |
dc.contributor.author | Park, Hee Ho | - |
dc.contributor.author | Park, Tai Hyun | - |
dc.date.accessioned | 2024-03-25T14:05:11Z | - |
dc.date.available | 2024-03-25T14:05:11Z | - |
dc.date.created | 2024-03-25 | - |
dc.date.issued | 2024-03 | - |
dc.description.abstract | Background: To improve the efficiency of neural development from human embryonic stem cells, human embryoid body (hEB) generation is vital through 3-dimensional formation. However, conventional approaches still have limitations: long-term cultivation and laborious steps for lineage determination. Methods: In this study, we controlled the size of hEBs for ectodermal lineage specification using cell-penetrating magnetic nanoparticles (MNPs), which resulted in reduced time required for initial neural induction. The magnetized cells were applied to concentrated magnetic force for magnet-derived multicellular organization. The uniformly sized hEBs were differentiated in neural induction medium (NIM) and suspended condition. This neurally induced MNP-hEBs were compared with other groups. Results: As a result, the uniformly sized MNP-hEBs in NIM showed significantly improved neural inductivity through morphological analysis and expression of neural markers. Signaling pathways of the accelerated neural induction were detected via expression of representative proteins; Wnt signaling, dopaminergic neuronal pathway, intercellular communications, and mechanotransduction. Consequently, we could shorten the time necessary for early neurogenesis, thereby enhancing the neural induction efficiency. Conclusion: Overall, this study suggests not only the importance of size regulation of hEBs at initial differentiation stage but also the efficacy of MNP-based neural induction method and stimulations for enhanced neural tissue regeneration. | - |
dc.identifier.bibliographicCitation | BIOMATERIALS RESEARCH, v.28 | - |
dc.identifier.doi | 10.34133/bmr.0011 | - |
dc.identifier.issn | 1226-4601 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/81799 | - |
dc.language | 영어 | - |
dc.publisher | American Association for the Advancement of Science (AAAS) | - |
dc.title | Improved Neural Inductivity of Size-Controlled 3D Human Embryonic Stem Cells Using Magnetic Nanoparticles | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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