dc.citation.conferencePlace |
KO |
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dc.citation.conferencePlace |
서울 |
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dc.citation.title |
KSBB 2018 International Academia-Industry Joint Meeting |
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dc.contributor.author |
Kim, Ye Eun |
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dc.contributor.author |
Kim, Chang Hee |
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dc.contributor.author |
Lee, Sung Kuk |
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dc.date.accessioned |
2024-02-01T01:11:32Z |
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dc.date.available |
2024-02-01T01:11:32Z |
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dc.date.created |
2019-01-09 |
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dc.date.issued |
2018-10-10 |
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dc.description.abstract |
Key factors for high production performance in biosynthetic process include continuous supply of redox cofactors for catalyzing reactions, which becomes critical in case of a pathway with redox imbalance. There are natural strains with high [NADPH]/[NADP+] reported. However, the lack of whole genome sequence or an inefficient genomic engineering tool makes it difficult to engineer such strains in contrast to a well-studied model organism Escherichia coli. The most distinguishing feature of their sugar metabolisms is the lack of functional Embden–Meyerhof–Parnas (EMP) pathway, which is a major glycolytic pathway in other organisms. E. coli mutants with no functional EMP pathway were reported to have higher [NADPH]/[NADP] than their wild type strains while showing growth defect on glucose as a sole carbon source. One of the strategies for acquiring mutants with desired phenotype is adaptive laboratory evolution (ALE). One of biosynthetic pathways producing platform chemical 3-hydroxypropionic acid (3-HP) from glucose requires two NADPH per a 3-HP produced. We applied ALE approach to obtain an E. coli mutant growing well without the functional EMP pathway. Genetic modifications in isolated mutants were identified to insight into the molecular mechanisms linking 3-HP biosynthesis. |
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dc.identifier.bibliographicCitation |
KSBB 2018 International Academia-Industry Joint Meeting |
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dc.identifier.uri |
https://scholarworks.unist.ac.kr/handle/201301/80822 |
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dc.language |
영어 |
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dc.publisher |
The Korean Society for Biotechnology and Bioengineering |
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dc.title |
Adaptive Laboratory Evolution Guided Engineering of Embden–Meyerhof–Parnas Pathway Disrupted Escherichia coli mutant and Its Application to Production of 3-Hydroxypropionic Acid |
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dc.type |
Conference Paper |
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dc.date.conferenceDate |
2018-10-10 |
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