dc.citation.conferencePlace |
KO |
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dc.citation.title |
한국화학공학회 2019년도 봄 총회 및 학술대회 |
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dc.contributor.author |
Thuan, Phu Nguyen-Vo |
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dc.contributor.author |
MUGECH SANKARANARAYANAN |
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dc.contributor.author |
Sankaranarayanan, Mugesh |
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dc.contributor.author |
Seol, Eunhee |
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dc.contributor.author |
Chun, Ah Yeong |
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dc.contributor.author |
Ashok, Somasundar |
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dc.contributor.author |
Chauhan, Ashish |
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dc.contributor.author |
Kim, Jung Rae |
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dc.contributor.author |
Ko, Seyoung |
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dc.contributor.author |
Kim, Donghyuk |
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dc.contributor.author |
Park, Sunghoon |
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dc.date.accessioned |
2024-02-01T00:36:14Z |
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dc.date.available |
2024-02-01T00:36:14Z |
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dc.date.created |
2020-01-15 |
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dc.date.issued |
2019-04-25 |
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dc.description.abstract |
3-Hydroxypropionic acid (3-HP) is an important platform chemical which can be converted to many useful chemicals. The toxic effects of 3-HP on cell growth and viability is a serious challenge for its industrial biological production. In this study, a highly 3-HP tolerant strain was developed from Escherichia coli W by adaptive laboratory evolution (ALE). Genome analysis indicated that 13 genes including glpK and yieP were mutated during ALE. For the mutated genes, contribution to 3-HP tolerance was studied by multiplex automated genome engineering (MAGE). Interestingly, a nonsense mutation in yieP alone was fully responsible for the improved tolerance to 3-HP. Mutation or complete deletion of yieP conferred a similar 3-HP tolerance to other E. coli strains. The study on 3-HP tolerance mechanism conferred by ΔyieP is in progress |
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dc.identifier.bibliographicCitation |
한국화학공학회 2019년도 봄 총회 및 학술대회 |
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dc.identifier.uri |
https://scholarworks.unist.ac.kr/handle/201301/79918 |
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dc.publisher |
한국화학공학회 |
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dc.title |
Deletion of a minor global regulator yieP greatly improves the tolerance of Escherichia coli to 3-hydroxypropionic acid |
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dc.type |
Conference Paper |
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dc.date.conferenceDate |
2019-04-24 |
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