dc.citation.conferencePlace |
KO |
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dc.citation.title |
KMB 46th Annual Meeting & international Symposium |
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dc.contributor.author |
Cho, Kyunghyun |
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dc.contributor.author |
Kim, Ye Eun |
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dc.contributor.author |
Lee, Sung Kuk |
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dc.date.accessioned |
2024-02-01T00:08:02Z |
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dc.date.available |
2024-02-01T00:08:02Z |
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dc.date.created |
2020-01-08 |
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dc.date.issued |
2019-06-23 |
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dc.description.abstract |
Recently, there has been an increasing interest in the production of bio-based platform chemicals as environmental problems and limited fossil resources issues have been highlighted. 3-Hydroxypropionic acid (3-HP) is a valuable bio-based platform chemical for the production of many other chemicals such as acrylic acid and malonic acid in the industry. As a number of efforts have been made to produce 3-HP using metabolically engineered microorganisms, the biosensors that sense the 3-HP levels inside cells will be used as a high-throughput screening tool. In this study, a transcriptional factor, originated from a Pseudomonas species, based 3-HP inducible biosensor that reports intracellular 3-HP level was successfully constructed in Escherichia coli MG1655. This study will be the starting point for the improvement of 3-HP biosensors. Further engineering of a promoter and 5’-UTR elements of the transcriptional factor encoding gene is under progress in order to enhance promoter strength and inducibility. |
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dc.identifier.bibliographicCitation |
KMB 46th Annual Meeting & international Symposium |
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dc.identifier.uri |
https://scholarworks.unist.ac.kr/handle/201301/79616 |
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dc.publisher |
The Korean Society for Microbiology and Biotechnology |
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dc.title |
Development of 3-Hydroxypropionic Acid Inducible Biosensor in Escherichia coli |
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dc.type |
Conference Paper |
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dc.date.conferenceDate |
2019-06-23 |
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