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DC Field | Value | Language |
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dc.citation.endPage | 6765 | - |
dc.citation.number | 18 | - |
dc.citation.startPage | 6756 | - |
dc.citation.title | CANCER RESEARCH | - |
dc.citation.volume | 64 | - |
dc.contributor.author | Do, Yoonkyung | - |
dc.contributor.author | Hegde, VL | - |
dc.contributor.author | Nagarkatti, PS | - |
dc.contributor.author | Nagarkatti, M | - |
dc.date.accessioned | 2023-12-22T10:43:42Z | - |
dc.date.available | 2023-12-22T10:43:42Z | - |
dc.date.created | 2014-10-08 | - |
dc.date.issued | 2004-09 | - |
dc.description.abstract | In this study, we investigated the effect of bryostatin-1 (Bryo-1), an antineoplastic agent, on dendritic cell (DC) maturation, activation, and functions. Murine bone marrow-derived DCs on culture with Bryo-1 alone, Bryo-1 + calcium ionophore (CI), but not CI alone exhibited morphologic changes characteristic of mature DCs and expressed increased levels of CD40, CD80, and CD86. Moreover, Bryo-1 + CI-treated DCs exhibited enhanced antigen-presenting ability to naive and antigen-specific T cells and alloreactive T cells. Bryo-1 + CI-mediated activation of DCs involved protein kinase C (PKC), especially PKC-α, δ, and - ι, and addition of PKC inhibitors impaired their ability to activate T cells. Bryo-1 + CI treatment of DCs did not activate mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase, p38 MAPK, or stress-activated protein kinase/c-Jun NH 2-terminal kinase pathways. Finally, treatment of DCs with Bryo-1 alone and Bryo-1 + CI, but not CI alone, induced nuclear translocation of nuclear factor κB as studied by confocal microscopy. DCs generated from human peripheral blood monocytes or from human cord blood CD34 + hematopoietic stem cells, when cultured with Bryo-1 + CI, also showed maturation and increased T-cell stimulatory activity. Bryo-1 + CI was more potent in inducing maturation and activation of DCs when compared with other agents such as tumor necrosis factor α, lipopolysaccharide, or phorbol 12-myristate 13-acetate + CI. Collectively, the current study shows for the first time that Bryo-1 alone or in combination with CI may promote the maturation of DCs and therefore may be useful in development of DC-based cancer immunotherapy. | - |
dc.identifier.bibliographicCitation | CANCER RESEARCH, v.64, no.18, pp.6756 - 6765 | - |
dc.identifier.doi | 10.1158/0008-5472.CAN-03-4002 | - |
dc.identifier.issn | 0008-5472 | - |
dc.identifier.scopusid | 2-s2.0-4644266896 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/7064 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=4644266896 | - |
dc.identifier.wosid | 000224089700057 | - |
dc.language | 영어 | - |
dc.publisher | AMER ASSOC CANCER RESEARCH | - |
dc.title | Bryostatin-1 enhances the maturation and antigen-presenting ability of murine and human dendritic cells | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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