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DC Field | Value | Language |
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dc.citation.endPage | 29 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 20 | - |
dc.citation.title | EUROPEAN JOURNAL OF IMMUNOLOGY | - |
dc.citation.volume | 38 | - |
dc.contributor.author | Do, Yoonkyung | - |
dc.contributor.author | Park, Chae Gyu | - |
dc.contributor.author | Kang, Young-Sun | - |
dc.contributor.author | Park, Sung Ho | - |
dc.contributor.author | Lynch, Rebecca M. | - |
dc.contributor.author | Lee, Haekyung | - |
dc.contributor.author | Powell, Bradford S. | - |
dc.contributor.author | Steinman, Ralph M. | - |
dc.date.accessioned | 2023-12-22T09:06:28Z | - |
dc.date.available | 2023-12-22T09:06:28Z | - |
dc.date.created | 2014-10-08 | - |
dc.date.issued | 2008-01 | - |
dc.description.abstract | There is a need for a more efficient vaccine against the bacterium Yersinia pestis, the agent of pneumonic plague. The Fl-LcrV (F1-V) subunit vaccine in alhydrogel is known to induce humoral immunity. In this study, we utilized DC to investigate cellular immunity. We genetically engineered the LcrV virulence protein into the anti-DEC-205/ CD205 mAb and thereby targeted the conjugated protein directly to mouse DEC-205+ DC in situ. We observed antigen-specific CD4+ T cell immunity measured by intracellular staining for IFN-γ in three different mouse strains (C57BL/6, BALB/c, and C3H/HeJ), while we could not observe such T cell responses with F1-V vaccine in alhydrogel. Using a peptide library for LcrV protein, we identified two or more distinct CD4+ T cell mimetopes in each MHC haplotype, consistent with the induction of broad immunity. When compared to nontargeted standard protein vaccine, DC targeting greatly increased the efficiency for inducing IFN-γ-producing T cells. The targeted LcrV protein induced antibody responses to a similar extent as the F1-V subunit vaccine, but Thl-dependent IgG2a and IgG2c isotypes were observed only after anti-DEC-205:LcrV mAb immunization. This study sets the stage for the analysis of functional roles of IFN-γ-producing T cells in Y. pestis infection. | - |
dc.identifier.bibliographicCitation | EUROPEAN JOURNAL OF IMMUNOLOGY, v.38, no.1, pp.20 - 29 | - |
dc.identifier.doi | 10.1002/eji.200737799 | - |
dc.identifier.issn | 0014-2980 | - |
dc.identifier.scopusid | 2-s2.0-38349052185 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/7056 | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/full/10.1002/eji.200737799 | - |
dc.identifier.wosid | 000252726300007 | - |
dc.language | 영어 | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | Broad T cell immunity to the LcrV virulence protein is induced by targeted delivery to DEC-205/CD205-positive mouse dendritic cells | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalResearchArea | Immunology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | CD205/DEC-205 | - |
dc.subject.keywordAuthor | cellular immunity | - |
dc.subject.keywordAuthor | dendritic cells | - |
dc.subject.keywordAuthor | LcrV | - |
dc.subject.keywordAuthor | Yersinia pestis | - |
dc.subject.keywordPlus | YERSINIA-PESTIS INFECTION | - |
dc.subject.keywordPlus | NECROSIS-FACTOR-ALPHA | - |
dc.subject.keywordPlus | V-ANTIGEN | - |
dc.subject.keywordPlus | PNEUMONIC PLAGUE | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | GAMMA-INTERFERON | - |
dc.subject.keywordPlus | FUSION PROTEIN | - |
dc.subject.keywordPlus | PROTECTION | - |
dc.subject.keywordPlus | VACCINE | - |
dc.subject.keywordPlus | UNRESPONSIVENESS | - |
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