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Author

Do, Yoonkyung
DC-based Immune System & Immunotherapy (DISNI) Lab
Research Interests
  • Study on various subsets of dendritic cells and their immunological functions

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Identification of antigen-presenting dendritic cells in mouse aorta and cardiac valves

Cited 91 times inthomson ciCited 87 times inthomson ci
Title
Identification of antigen-presenting dendritic cells in mouse aorta and cardiac valves
Author
Choi, Jae-HoonDo, YoonkyungCheong, CheolhoKoh, HyeinBoscardin, Silvia B.Oh, Yong-SeokBozzacco, LeoniaTrumpfheller, ChristinePark, Chae GyuSteinman, Ralph M.
Keywords
ARTERIAL INTIMA; ATHEROSCLEROTIC LESIONS; AIRWAY INFLAMMATION; ADAPTIVE IMMUNITY; RAT-LIVER; T-CELLS; ACCUMULATION; EXPRESSION; RESPONSES; PLAQUES
Issue Date
200905
Publisher
Society for Experimental Stroke
Citation
JOURNAL OF EXPERIMENTAL STROKE AND TRANSLATIONAL MEDICINE, v.206, no.3, pp.497 - 505
Abstract
Presumptive dendritic cells (DCs) bearing the CD11c integrin and other markers have previously been identified in normal mouse and human aorta. We used CD11c promoter-enhanced yellow fluorescent protein (EYFP) transgenic mice to visualize aortic DCs and study their antigen-presenting capacity. Stellate EYFP + cells were readily identified in the aorta and could be double labeled with antibodies to CD11c and antigen-presenting major histo-compatability complex (MHC) II products. The DCs proved to be particularly abundant in the cardiac valves and aortic sinus. In all aortic locations, the CD11c + cells localized to the subintimal space with occasional processes probing the vascular lumen. Aortic DCs expressed little CD40 but expressed low levels of CD1d, CD80, and CD86. In studies of antigen presentation, DCs selected on the basis of EYFP expression or binding of anti-CD11c antibody were as effective as DCs similarly elected from the spleen. In particular, the aortic DCs could cross-present two different protein antigens on MHC class I to CD8 + TCR transgenic T cells. In addition, after intravenous injection, aortic DCs could capture anti-CD11c antibody and cross-present ovalbumin to T cells. These results indicate that bona fide DCs are a constituent of the normal aorta and cardiac valves.
URI
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DOI
http://dx.doi.org/JOURNAL OF EXPERIMENTAL MEDICINE
ISSN
1939-067X
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