dc.citation.endPage |
939 |
- |
dc.citation.number |
3 |
- |
dc.citation.startPage |
931 |
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dc.citation.title |
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS |
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dc.citation.volume |
217 |
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dc.contributor.author |
Han, JK |
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dc.contributor.author |
Lee, Sung Kuk |
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dc.date.accessioned |
2023-12-22T12:42:21Z |
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dc.date.available |
2023-12-22T12:42:21Z |
- |
dc.date.created |
2014-09-29 |
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dc.date.issued |
1995-03 |
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dc.description.abstract |
In this report, we investigated the possibility that a product of phosphatidylinositol 4,5-bisphosphate (PIP2) breakdown, inositol 1,4,5-trisphosphate (Ins(1,4,5)P-3), and its downstream effector, Ca2+, is involved in oocyte maturation. Microinjection of monoclonal antibody specifically directed to PIP2 into oocytes prior to progesterone addition inhibited meiotic maturation. In addition, preventing or suppressing the progesterone-induced increase in [Ca2+](i) and maintaining cytosolic free Ca2+ of oocyte at 0.1 or 0.3 mu M by Br-2-BAPTA buffered Ca2+ solutions, completely blocked maturation. However, raising cytosolic Ca2+ concentration of oocyte to 1.0 or 3.0 mu M, the inhibitory Br-2-BAPTA effects on oocyte maturation was greatly diminished. Finally, microinjection of heparin, a potent antagonist of Ins(1,4,5)P-3 receptor, inhibited progesterone-induced oocyte maturation in a manner that is linearly proportional to its cytoplasmic concentration. These results strongly support the hypothesis that PIP2 turnover as well as Ins(1,4,5)P-3-induced Ca2+ release play crucial roles in regulating normal meiotic cell division in Xenopus oocyte. |
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dc.identifier.bibliographicCitation |
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.217, no.3, pp.931 - 939 |
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dc.identifier.doi |
10.1006/bbrc.1995.2860 |
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dc.identifier.issn |
0006-291X |
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dc.identifier.scopusid |
2-s2.0-0029417187 |
- |
dc.identifier.uri |
https://scholarworks.unist.ac.kr/handle/201301/6789 |
- |
dc.identifier.url |
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0029417187 |
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dc.identifier.wosid |
A1995TM60200031 |
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dc.language |
영어 |
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dc.publisher |
ACADEMIC PRESS INC ELSEVIER SCIENCE |
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dc.title |
Reducing PIP2 hydrolysis, Ins(1,4,5)P-3 receptor availability, or calcium gradients inhibits progesterone-stimulated Xenopus oocyte maturation |
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dc.type |
Article |
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dc.description.journalRegisteredClass |
scopus |
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