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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 1657 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1650 | - |
dc.citation.title | ACS CENTRAL SCIENCE | - |
dc.citation.volume | 9 | - |
dc.contributor.author | Mishra, Pratyush Kumar | - |
dc.contributor.author | Sharma, Nirmali | - |
dc.contributor.author | Kim, Hyunwoo | - |
dc.contributor.author | Lee, Changwook | - |
dc.contributor.author | Rhee, Hyun-Woo | - |
dc.date.accessioned | 2023-12-21T11:51:26Z | - |
dc.date.available | 2023-12-21T11:51:26Z | - |
dc.date.created | 2023-08-14 | - |
dc.date.issued | 2023-08 | - |
dc.description.abstract | GEN-Click is a genetically encoded copper-click reaction.It enables spatial biotin labeling of the azide or alkyne-tagged biomoleculesin live cells. Chemical reactions for the in situ modificationof biomolecules within living cells are under development. Among thesereactions, bio-orthogonal reactions such as click chemistry usingcopper(I) and Staudinger ligation are widely used for specific biomoleculetracking in live systems. However, currently available live cell copper(I)-catalyzedazide/alkyne cycloaddition reactions are not designed in a spatiallyresolved manner. Therefore, we developed the "GEN-Click"system, which can target the copper(I)-catalyzed azide/alkyne cycloadditionreaction catalysts proximal to the protein of interest and can begenetically expressed in a live cell. The genetically controlled,spatially restricted, metal-catalyzed biorthogonal reaction can beused for proximity biotin labeling of various azido-bearing biomolecules(e.g., protein, phospholipid, oligosaccharides) in living cell systems.Using GEN-Click, we successfully detected local metabolite-transferringevents at cell-cell contact sites. | - |
dc.identifier.bibliographicCitation | ACS CENTRAL SCIENCE, v.9, no.8, pp.1650 - 1657 | - |
dc.identifier.doi | 10.1021/acscentsci.3c00511 | - |
dc.identifier.issn | 2374-7943 | - |
dc.identifier.scopusid | 2-s2.0-85167888838 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/65172 | - |
dc.identifier.wosid | 001033263900001 | - |
dc.language | 영어 | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | GEN-Click: Genetically Encodable Click Reactions for Spatially Restricted Metabolite Labeling | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.type.docType | Article; Early Access | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | CHEMISTRY | - |
dc.subject.keywordPlus | AZIDES | - |
dc.subject.keywordPlus | CELL | - |
dc.subject.keywordPlus | BIOCONJUGATION | - |
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