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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 219 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 214 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 7 | - |
dc.contributor.author | Cheong, NE | - |
dc.contributor.author | Choi, YO | - |
dc.contributor.author | Kim, WY | - |
dc.contributor.author | Bae, IS | - |
dc.contributor.author | Cho, Moo Je | - |
dc.contributor.author | Hwang, I | - |
dc.contributor.author | Kim, JW | - |
dc.contributor.author | Lee, SY | - |
dc.date.accessioned | 2023-12-22T12:37:34Z | - |
dc.date.available | 2023-12-22T12:37:34Z | - |
dc.date.created | 2014-09-22 | - |
dc.date.issued | 1997-04 | - |
dc.description.abstract | A 28-kDa antifungal PR-5 protein (PLTP) was purified from pumpkin leaves to homogeneity by using ammonium sulfate fractionation, a regenerated chitin column, and reversed-phase column chromatographies on butyl-Toyopearl and HPLC C18 columns. Analysis of 14 N-terminal amino acid sequences of PLTP shows 100% sequence identity to those of two PR-5 proteins, NP24 from tomatoes and AP24 from tobacco. The identical sequence also exhibited high amino acid sequence homology to that of an osmotin-like protein (OLP; 71%) from tobacco cells and thaumatin (64%), a sweet-tasting protein of Thaumatococcus danielli Bench. When the PLTP was immuno-blotted with antiserum raised against the tobacco OLP, the OLP antibody specifically cross-reacted with the PLTP, suggesting that they share several common epitopes in their tertiary structure of the proteins. The purified PLTP rapidly lyzed hyphal tips of Neurospora crassa at a concentration greater than 200 nM and significantly inhibited the fungal growth of Fusarium oxysporum in an agar-disc plate at a concentration greater than 2 μM. It also shows a synergistic effect with nikkomycin, a chitin synthase inhibitor, for the growth inhibition of Candida albicans. | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.7, no.2, pp.214 - 219 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.scopusid | 2-s2.0-0031591022 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/6377 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0031591022 | - |
dc.identifier.wosid | A1997WX19900011 | - |
dc.language | 영어 | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.title | Purification and characterization of an antifungal PR-5 protein from pumpkin leaves | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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