BROWSE

Related Researcher

Author

Cho, Moo Je
Ulsan National Institute of Science and Technology
Research Interests
  • Calcium Signaling

ITEM VIEW & DOWNLOAD

Molecular characterization of a rab-related small GTP binding protein cDNA from rice (Oryza sativa L IR-36)

Cited 4 times inthomson ciCited 4 times inthomson ci
Title
Molecular characterization of a rab-related small GTP binding protein cDNA from rice (Oryza sativa L IR-36)
Author
Kim, HYSeo, HSJeong, JYLee, SYCho, Moo JeBahk, JD
Keywords
RAPID ISOLATION; EXPRESSION; GENES; ARABIDOPSIS; SECRETION; MEMBRANE; PRODUCTS; CELLS; YPT1
Issue Date
199704
Publisher
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
Citation
MOLECULES AND CELLS, v.7, no.2, pp.226 - 230
Abstract
To study physiological roles of plant small GTP binding proteins, we isolated a cDNA clone (ORrab2) encoding the rab-related small GTP binding protein from rice (Oryza sativa L. IR-36) by using human cDNA rab2 as a probe. The deduced amino acid sequence of the ORrab2 gene shared all the conserved regions, important for GTPase/GTP binding activities, with those of other small GTP binding proteins. ORrab2 is a 1028 bp long cDNA, encodes a 23.2 kDa protein which shows 85.2% similarity on the amino acid sequence level to the Hrab2 protein, and was used as a probe. Through Southern and Northern blot analyses, we found that ORrab2 is a single copy gene and actively expressed at the stages of cell division and elongation. We investigated GTP binding abilities by a filter assay procedure. Deletion of a binding motif, GDTGVGKS, within an ORrab2 protein showed a significant decrease of GTP binding affinity, suggesting its important role in nucleotide binding.
URI
Go to Link
ISSN
1016-8478
Appears in Collections:
SLS_Journal Papers

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qr_code

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU