Molecular characterization of a rab-related small GTP binding protein cDNA from rice (Oryza sativa L IR-36)
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- Molecular characterization of a rab-related small GTP binding protein cDNA from rice (Oryza sativa L IR-36)
- Kim, HY; Seo, HS; Jeong, JY; Lee, SY; Cho, Moo Je; Bahk, JD
- RAPID ISOLATION; EXPRESSION; GENES; ARABIDOPSIS; SECRETION; MEMBRANE; PRODUCTS; CELLS; YPT1
- Issue Date
- KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
- MOLECULES AND CELLS, v.7, no.2, pp.226 - 230
- To study physiological roles of plant small GTP binding proteins, we isolated a cDNA clone (ORrab2) encoding the rab-related small GTP binding protein from rice (Oryza sativa L. IR-36) by using human cDNA rab2 as a probe. The deduced amino acid sequence of the ORrab2 gene shared all the conserved regions, important for GTPase/GTP binding activities, with those of other small GTP binding proteins. ORrab2 is a 1028 bp long cDNA, encodes a 23.2 kDa protein which shows 85.2% similarity on the amino acid sequence level to the Hrab2 protein, and was used as a probe. Through Southern and Northern blot analyses, we found that ORrab2 is a single copy gene and actively expressed at the stages of cell division and elongation. We investigated GTP binding abilities by a filter assay procedure. Deletion of a binding motif, GDTGVGKS, within an ORrab2 protein showed a significant decrease of GTP binding affinity, suggesting its important role in nucleotide binding.
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