BROWSE

Related Researcher

Author

Cho, Moo Je
Ulsan National Institute of Science and Technology
Research Interests
  • Calcium Signaling

ITEM VIEW & DOWNLOAD

Two hevein homologs isolated from the seed of Pharbitis nil L. exhibit potent antifungal activity

Cited 84 times inthomson ciCited 79 times inthomson ci
Title
Two hevein homologs isolated from the seed of Pharbitis nil L. exhibit potent antifungal activity
Author
Koo, JCLee, SYChun, HJCheong, YHChoi, JSKawabata, SMiyagi, MTsunasawa, SHa, KSBae, DWHan, CDLee, BLCho, Moo Je
Keywords
(Pharbitis nil); Hevein homologous antimicrobial peptide
Issue Date
199801
Publisher
Elsevier BV
Citation
BIOCHIMICA ET BIOPHYSICA ACTA - PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, v.1382, no.1, pp.80 - 90
Abstract
Two antifungal peptides (Pn-AMP1 and Pn-AMP2) have been purified to homogeneity from seeds of Pharbitis nil. The amino acid sequences of Pn-AMP1 (41 amino acid0 residues) and Pn-AMP2 (40 amino acid residues) were identical except that Pn-AMP1 has an additional serine residue at the carboxyl-terminus. The molecular masses of Pn-AMP1 and Pn-AMP2 were confirmed as 4299.7 and 4213.2Da, respectively, Both the Pn-AMPs were highly basic (pI 12.02) and had characteristics of cysteine/glycine rich chitin-binding domain. Pn-AMPs exhibited potent antifungal activity against both chitin-containing and non-chitin-containing fungi in the cell wall. Concentrations required for 50% inhibition of fungal growth were ranged from 3 to 26 mu g/ml for Pn-AMP1 and from 0.6 to 75 mu g/ml for Pn-AMP2. The Pn-AMPs penetrated very rapidly into fungal hyphae and localized at septum and hyphal tips of fungi, which caused burst of hyphal tips. Burst of hyphae resulted in disruption of the fungal membrane and leakage of the cytoplasmic materials. To our knowledge, Pn-AMPs are the first hevein-like proteins that show similar fungicidal effects as thionins do.
URI
Go to Link
DOI
http://dx.doi.org/10.1016/S0167-4838(97)00148-9
ISSN
0167-4838
Appears in Collections:
SLS_Journal Papers

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qr_code

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU